鸡胚发育过程四种放射状胶质细胞标记物的对比分析  

Expression comparison of four different markers for radial glial cells

作　　者：杜蕊[1] 杨慈清[2] 李玲玲[1] 段明慧[1] 卢习[1] 陈树林[1] 赵善廷[1] 

机构地区：[1]西北农林科技大学动物医学院,杨陵712100 [2]新乡医学院生命科学技术学院,新乡453000

出　　处：《中国免疫学杂志》2013年第11期1207-1212,1215,共7页Chinese Journal of Immunology

基　　金：西北农林科技大学人才专项基金(No.Z111021101)

摘　　要：目的:比较脑脂结合蛋白(Brain lipid binding protein,BLBP)、放射状胶质细胞标记物2(Radial glial cell marker-2,RC2)、巢蛋白(Nestin)和胶质纤维酸性蛋白(Glial fibrillary acidic protein,GFAP)在鸡胚不同发育时期视顶盖胶质细胞(Glial cell,GC)中表达的差异。方法:从鸡胚发育的第8天(E8)开始到E18,分别进行取材、固定、包埋,振荡切片,采用免疫荧光技术对不同发育时期的鸡胚视顶盖的胶质细胞进行标记,同时采用活体原位电转的方法对E8、E10和E12的放射状胶质细胞(Radial glial cells,RGCs)进行标记。结果:从E8开始,BLBP、RC2、Nestin和GFAP均开始表达。BLBP和GFAP抗体标记GC从E8到E18均可表达,BLBP标记的细胞从E14开始出现变化,呈现迁移中神经元的特点,GFAP标记的细胞在E18开始出现变化,呈现星形胶质细胞形态。自E8-E16,RC2和Nestin标记RGCs,但E18时标记RGCs已不明显。结论:4种抗体均可标记E8-E18的GC,在E12之前4种抗体标记RGCs效果相似,而在E12后RC2,Nestin与BLBP标记RGCs开始出现差异,从E18开始GFAP开始标记星形胶质细胞(Astrocyte)。Objective： To compare the difference of four different markers for radial glial cells： Brain lipid binding protein（ BLBP）,radial glial cell marker-2（ RC2）,Nestin and glial fibrillary acidic protein（ GFAP） in chicken embryonic optic tectum at different developmental stages. Methods： Tissues were harvested from E8 to E18 and fixed with 4% PFA,embeded with agarose,then cut with vibratome. Immunofluorescence technique was used to stain the RGCs in chicken optic tectum at different developmental stages.Radial glial cells were labeled by electroporation in ovo at E8,E10 and E12. Results： BLBP,RC2,Nestin and GFAP started to express from E8. GCs could be labeled by BLBP and GFAP from E8 to E18. The cells labeled by BLBP began to change from E14,showing the morphological characteristics of migrating neuron. The cells labeled by GFAP began to change in E18,showing morphological characteristics of astrocytes. RGCs were labeled by RC2 and Nestin from E8 to E16. RGCs are labeled by RC2 and Nestin had not been evident at E18. Conclusion： RGCs are labeled by these 4 antibodies from E8 to E18. The effects of labeling RGCs are similar before E12. While RGCs are labeled by RC2,Nestin and BLBP begin to change after E12. Astrocytes begin to be labeled by GFAP at E18.

关 键 词：鸡胚胎 视顶盖 放射状胶质细胞 星形胶质细胞 免疫荧光技术 

分 类 号：Q954.48[生物学—动物学]