抑制消减杂交技术(SSH)及其在兽医领域的应用  

Suppression subtractive hybridization (SSH) technique and its application in the veterinary field

作　　者：孟庆峰[1] 刘琪[2] 王伟利[1] 

机构地区：[1]吉林出入境检验检疫局,长春130062 [2]吉林农业大学动物科技学院,长春130118

出　　处：《黑龙江畜牧兽医》2013年第12期23-26,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家质检总局科技计划项目(2009B151;2009IK168;2012IK010);吉林省科技发展计划项目(20090249;201205052)

摘　　要：基因的差异表达是生命活动的核心机制,在不同发育时期和不同生理阶段中不同细胞或同类细胞在表达上不同,这为研究生命活动过程提供重要的信息。抑制消减杂交技术(SSH)是近年来新兴的用于分离差异表达基因的手段之一,此技术结合了消减杂交和抑制性PCR的优点,广泛应用于兽医领域。文章对SSH原理、方法和特点进行了阐述,对其在微生物、动物疾病、动物生育和寄生虫学中的应用做了相应的概括介绍。The differential expression of genes is the core mechanism of life activities. Because different cells or similar ceils are not the same in gene expression at different developmental and physiological stages, this provides important information for the study of biological processes. Suppression subtractive hybridization （SSH）＇ is one of the means to separate differentially expressed genes emerging in recent years, this tech- nique combines the advantages of suppression subtractive hybridization and suppression PCR, which is widely used in the veterinary field. In this paper, the principles, methods and characteristics of SSH technique are described, and the applications of SSH technique in microbiology, animal diseases, animal fertility and parasitology are correspondingly generalized and described.

关 键 词：抑制消减杂交技术(SSH) 差异表达 兽医领域 

分 类 号：Q786[生物学—分子生物学]