羊衣原体的分离鉴定及PCR诊断方法的建立  

Isolation and identification of ovine chlamydophila abortus and the establishment of PCR diagnostic method

作　　者：王光华[1] 叶成玉[1] 周继章[2] 陆艳[1] 马利青[1] 

机构地区：[1]青海省畜牧兽医科学院,西宁810016 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室/农业部兽医公共卫生重点开放实验室,兰州730046

出　　处：《黑龙江畜牧兽医》2013年第12期104-106,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：现代农业产业技术体系建设专项(CARS-40-09B);青海省省校联合项目(QNH-2010-395)

摘　　要：为了研究青海省乌兰县牧户母羊流产的病因,试验采用临床症状观察、显微镜观察、鸡胚培养和PCR检测等方法对病原进行了鉴定,并建立衣原体PCR诊断方法对病原体进行了敏感性和稳定性检测。结果表明:引起母羊流产的病原为羊流产嗜性衣原体。说明建立的PCR方法能够对羊衣原体病原作出正确的检测,可用于羊衣原体病的实验室诊断。To study the cause of aborted lambs belonged to the herdsmen in Wulan county of Qinghai province, the methods including the obser- vations of clinical symptoms and microscope, chick embryo cultivation and PCR detection, were used to identify the pathogen. The sensitivity and stability tests for the pathogen were performed based on the PCR method which was established for the detection of Chlamydophila. The re- suits showed that the pathogen causing abortion in ewes was identified as ovine Chlamydophila abortus. The results indicate that the established PCR method can correctly detect the pathogen of ovine Chlamydophila, which can be used for the laboratory diagnosis of ovine chlamydiosis.

关 键 词：羊衣原体病 分离 鉴定 MOMP基因 

分 类 号：S852.67[农业科学—基础兽医学]