传染性囊病病毒感染雏鸡外周血液T细胞亚型数量及增殖功能变化  

Changes of the T cell subtypes quantity and their proliferations in peripheral blood of chicks infected with IBDV

作　　者：隋欣[1] 郑艳生[1] 高雪丽[1] 郑世民[1] 吕晓萍[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2013年第11期3-5,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金青年基金项目(30901062);东北农业大学科学研究基金资助项目

摘　　要：为探讨IBDV鸡后对机体外周血细胞和体液免疫功能的影响,深入研究IBD免疫致病机制,并为其有效防控提供科学依据,本研究以21日龄雏鸡为研究对象,应用细胞培养技术及MTT法、ANAE法、流式细胞术等方法,对感染IBDV雏鸡外周血液T细胞增殖功能、TANAE^+细胞数量、CD4^+T细胞CD8^+T细胞数量进行检测,结果发现,雏鸡感染IBDV后1~42d外周血液TANAE^+细胞数量、T淋巴细胞增殖功能、CD4^+T细胞数量、CD8^+T细胞数量均有不同程度的降低。结果表明,雏鸡感染IBDV后,外周血液细胞免疫功能受到抑制,抗病毒能力降低。To discuss the cellular and humoral immunity function in peripheral blood of chicks infected with IBDV, cell culture and MTI＂, histoehemical staining, flow cytometry, the proliferative function of T lymphocyte were used to examine the number of TA- NAE＋ cells, the number of CD4＋T ceils and CD8＋ T cells in peripheral blood of 21-day-age chickens infected with IBDV .The results show that the number of TANAE＋ cells , CD4＋T cells and CD8~ T cells, the proliferative function of T lymphocytes were reduced after infecting with IBDV 1 to 42 days. These results indicated that the function of cellular immunity in peripheral blood of chickens infect- ed with IBDV was reduced, which comporomises the response of chickens to diseases.

关 键 词：鸡 IBDV 外周血液 细胞免疫 

分 类 号：S858.39[农业科学—临床兽医学]