江苏地区火鸡组织滴虫18S rRNA基因的克隆及系统发育分析  

Cloning and Phylogenetic Analysis of the 18S rRNA of Histomonas Meleagridis from Jiangsu Province

作　　者：刘聪[1,2] 曲昌宝[1,2] 郭平[1,2] 李聪[1,2] 张祖航[1,2] 陶建平[1,2] 许金俊[1,2] 

机构地区：[1]江苏动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009 [2]扬州大学兽医学院禽类预防医学教育部重点实验室,江苏扬州225009

出　　处：《中国兽医杂志》2013年第11期9-12,共4页Chinese Journal of Veterinary Medicine

基　　金：江苏省大学生实践创新训练计划(2012JSSPITP1357);江苏省基础研究计划(自然科学基金)面上项目(BK20131229);扬州大学“新世纪人才工程”项目(2012);江苏高校兽医学优势学科资助;教育部创新团队计划(IRT0978)

摘　　要：为从分子水平了解江苏地区火鸡组织滴虫的分类地位和特征,本研究以江苏地区感染火鸡组织滴虫的发病鸡群的病变肝组织为材料,通过DNA提取、PCR扩增、DNA片段的克隆及测序,获得了江苏地区火鸡组织滴虫11个18S rRNA序列,并与GenBank上登录的其他地方火鸡组织滴虫和相关虫体18S rRNA基因序列进行了系统发育分析.结果表明,江苏地区火鸡组织滴虫18S rRNA基因同源性在99.0％～99.8％之间,亲缘关系较近,但形成2个分支,可能存在不同的基因型.研究结果为火鸡组织滴虫的分子诊断、分子流行病学调查以及种群遗传学的进一步研究奠定了基础.To identify the taxonomic status of Histomonas meleagridis in Jiangsu province, eleven liver samples of chicken which were diagnosed as Histomonas meleagridis infection were collected, sequences of 18S rRNA gene of Histomonas meleagridis were obtained by polymerase chain reaction, cloning and sequencing. The homology of 18S rRNA gene was analyzed and the phyloge- netic tree was constructed. Results indicated that the sequence similarity of 18S rRNA gene was in the range of 99.0%-99.8%, which formed two different phylogenetic branches. Different genotypes of Histomonas meleagridis in Jiangsu province may exist. This study laid a foundation for the detection, molecular epidemiology investigation and study of population genetics of Histomonas meleagridis.

关 键 词：火鸡组织滴虫 18 SrRNA 系统发育树 

分 类 号：S858.39[农业科学—临床兽医学]