猪流行性腹泻病毒PEDV-GD12株的分离与鉴定  

Isolation and Identification of Porcine Epidemic Diarrhea Virus PEDV-GD12 strain

作　　者：古洪浪[1] 邓胜朝 崔进[1] 刘晓露 刘超 袁航[3] 马岚[3] 张桂红[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]深圳市海王英特龙生物技术股份有限公司,广东深圳518055 [3]清华大学深圳研究生院,广东深圳518055

出　　处：《中国兽医杂志》2013年第11期22-25,共4页Chinese Journal of Veterinary Medicine

基　　金：现代农业产业技术体系建设专项资金资助(CARS-36)

摘　　要：采集广东省某猪场腹泻仔猪的小肠病料,通过在Vero细胞上培养,RT-PCR及间接免疫荧光检测,电镜观察等方法鉴定,确定分离到一株PEDV毒株,并命名为PEDV-GD12。通过对PEDV M基因的克隆测序以及进化分析,显示所分离到的毒株近年来流行的毒株同源性很高,处在同一分支上。A porcine epidemic diarrhea virus （PEDV） designated PEDV-GD12 was isolated from the small intestine feces of diarrheaed piglets in Guangdong province and cultured in Veto cell.The isolated virus was identified by RT-PCR,immunofluorescence,electronic microscope etc.The M gene of PEDV was amplified and sequenced and namecl by PEDV-GD 12.Phytogenetic analysis showed that the isolated strain shares a high homology with the pandemic strains in recent years and they are clustered into the same branch.

关 键 词：PEDV 分离鉴定 M基因 进化分析 

分 类 号：S852.651[农业科学—基础兽医学]