仔猪睾丸间质细胞培养方法的比较  

The Comparison of Culture Methods of Piglet Leydig Cells

作　　者：卿利娟[1] 魏泓[2] 李婧[1] 肖榕[1] 吴群[1] 葛亮[1] 商海涛[2] 郑小波[1] 刘宇[2] 

机构地区：[1]西南大学动物科技学院,重庆北碚400715 [2]第三军医大学基础部实验动物学教研室,重庆沙坪坝400038

出　　处：《中国兽医杂志》2013年第11期29-31,35,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然青年科学基金(31000546);国家高技术研究发展计划(863计划)(2012AA020603);国家科技支撑计划课题(2012BAI39B04)

摘　　要：为了对比仔猪睾丸间质细胞不同培养方法的培养效果。运用过筛培养法、不过筛培养法,组织块培养法3种方法对仔猪睾丸间质细胞进行体外分离和培养,并对其进行细胞各项指标的比较。结果显示,不过筛培养法培养的细胞活力、纯度、细胞功能都较好,培养时间也短;过筛培养法培养的细胞活力较低;组织块培养法培养的细胞纯度较低。但在其他细胞指标上,后两种方法又各自有优点。结果3种培养方法都能简单有效的培养出状态良好的仔猪睾丸间质细胞,但其在纯度,活力,培养时间等方面有所差别。Objective To compare the effect of different culture methods on the growth of piglet leydig cells. Methods Piglet ley- dig cells were isolated and cultured by three methods, steel net filtration, non-steel ＇net filtration and primary tissue explant culture. The characteristics of cells cultured by different methods were identified with different kinds of cell index. Results For the method of steel net filtration, the cell viability was a little low ; the method of non-steel net filtration was good ; for the method of primary tissue explant culture, the culture time and the cell purity were weak. Conclusion The three methods are easy and efficient for the culture of piglet teydig cells, but these methods have some differences in the aspects of cells purity, viability and culture time.

关 键 词：睾丸间质细胞 猪 纯度 活力 功能 

分 类 号：S828[农业科学—畜牧学]