叶尔羌高原鳅表皮抗菌肽的提取及抑菌试验  

Extraction Of Antimicrobial Peptides From Epidermis of Triplophysa Yarkandensis And Bacteriostasis Experiment

作　　者：贾山岭[1] 刘书东[1] 潘伊微[1] 陈根元[2] 李莲瑞[2] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]塔里木畜牧科技兵团重点实验室,新疆阿拉尔843300

出　　处：《中国兽医杂志》2013年第11期36-38,I0001,共4页Chinese Journal of Veterinary Medicine

基　　金：新疆生产建设兵团博士基金(2009 JC18)

摘　　要：生物体产生的抗菌肽是对抗外源性病原体侵袭致病作用的防御性肽类活性物质。本试验主要取活体叶尔羌高原鳅的表皮放入研钵中,加入蛋白酶抑制剂和Tris--c1研成肉糊状,用乙酸乙酯浸提法提取抗菌肽;经水合茚三酮显色反应为蓝紫色,DE-52纯化产物后SDS-PAGE蛋白电泳鉴定,其分子量约为9 700Da,并对大肠杆菌和金黄色葡萄球菌表现出明显的抗菌活性。Antimicrobial peptides are defensive peptide active substances produced by organisms and work against the inva- sion of exogenous pathogens. In this study, the skin of the Triplophysa （Hedinichthys） yarkandensis（Day） was collected in vivo and placed in a mortar, protease inhibitors and Tris-cl were added into the paste, and antimicrobial peptides were extracted with ethyl ac- etate extraction;The hydration reaction of ninhydrin gave a blue purple color, after DE-52 purification, the protein was identified by SDS-PAGE electrophoresis, and its molecular weight was about 9 700Da. This peptide showed significant antimicrobial activity against Escherichia coli and Staphylococcus aureus.

关 键 词：叶尔羌高原鳅 抗菌肽 抑菌试验 

分 类 号：S917.4[农业科学—水产科学]