青钱柳茎段腋芽的离体培养技术  

In vitro culture technique of axillary buds from stem of Cyclocarya paliurus

作　　者：鲁萌[1] 阮氏钏[1] 王纪[1] 方升佐[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2013年第6期6-10,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家林业局林业科学技术推广项目([2011]18号);国家林业公益性行业科研专项项目(20090406)

摘　　要：为建立高效的青钱柳快速繁殖体系,以青钱柳茎段腋芽为外植体,WPM培养基为基本培养基,研究了不同植物生长调节剂对青钱柳腋芽萌发、丛生芽增殖及生根诱导的影响。结果表明:在腋芽萌发过程中,9个组合处理对腋芽萌发均有促进作用,其中5 mg/L 6-BA+1.5 mg/L KT+0.1 mg/L NAA为最佳组合,腋芽萌芽率86.67%;腐胺具有促进腋芽萌动,缩短萌芽周期的作用;丛生芽增殖培养中,以0.3 mg/L TDZ+0.3 mg/L 6-BA增殖效果最佳,增殖系数为6.78;生根诱导中,10 mg/L GGR-6+1.0 mg/L IBA组合处理效果最好,生根率为25%,生长状态优于其他处理。To establish an efficient tissue culture system for rapid propagation of Cyclocarya paliurus, axillary buds from the stem segment were used as explants and cultured on WPM medium. Effects of the different types and concentrations of plant growth regulators on the germination of axillary buds, proliferation of multiple shoots and rooting induction were examined. The results showed that all nine hormone combinations promoted germination of axillary buds to some extent and the most suitable hormone combination was 5 mg/L 6 - BA ＋ 1.5 mg/L KT ＋ 0.1 mg/L NAA, the germination rate achieved 86.67% at the aforesaid conditions. Putrescine could promote axillary bud germination and shorten the cycle of germination. The combination of 0.3 mg/L TDZ ＋ 0.3 mg/L 6 - BA was the best treatment for the culture of shoot in- duction, and the multiplication coefficient could reach 6.78. The treatment of 10mg/L GGR - 6 ＋ 1.0 mg/L IBA had the best effect on rooting induction, and its rooting rate was 25% with superior growth performance.

关 键 词：青钱柳 植物生长调节剂 腋芽 增殖系数 生根诱导 

分 类 号：S723.13[农业科学—林木遗传育种]