植物体细胞胚发生胚性潜势恢复的研究进展  

Research progress on the reacquisition of embryogenic potentiality in plant somatic embryogenesis

作　　者：甄艳[1] 陈金慧[1] 施季森[1] 

机构地区：[1]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2013年第6期147-152,共6页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏省高校自然科学重大研究项目(12KJA220002);"十二五"国家科技支撑计划(2012BAD01B05);博士点基金优先发展领域计划(20113204130002);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：植物体细胞胚胎发生(简称体胚发生)是细胞全能性的体现。在适宜的培养体系下,植物的体细胞理论上均存在脱分化启动胚发育进程的可能。尽管体胚发生这一生物学发育进程的报道已有50多年的历史,但仍有一些重要的科学问题还未得到完全阐明,例如成熟外植体胚性能力恢复的机理、外源/内源因子如何调控胚性能力获得、胚性获得的关键分子途径、胚性获得调控的信号网络等。笔者综述了体胚能力恢复的诱导因子、生理生化标记、调控基因、miRNA调控和蛋白质组特征等方面的研究进展。Plant somatic embryogenesis is the manifestation of cell totipotency. Somatic cell initiates embryo develop- ment under appropriate conditions. Although this phenomenon has been reported for more than fifty years, some details are not clear, such as mechanism of embryogenic reacquisition from mature explants, exogenous/endogenous factors how to regulate embryogenic reacquisition, the key molecular steps of embryogenic reacquisition, regulation of signal network during embryogenic reacquisition. Acquisition of embryogenic competence is an interesting scientific question. This arti- cle reviews inducible factors of embryogenic competence, physiological and biochemical markers of embryogenic compe- tence and specific genes, miRNA regulation and proteins related to embryogenic competence.

关 键 词：愈伤组织 胚性能力 植物体胚发生 

分 类 号：S722[农业科学—林木遗传育种] Q948[农业科学—林学]