野百合与金佛山百合的核型分析及45S rDNA在其染色体上的物理定位  

The karyotype analysis and physical localization of 45S rDNA on the chromosomes of Lilium brownii and L. jinftishanense

作　　者：席梦利[1] 房磊[1] 张晓玲[1] 辛昊阳 高婷婷[1] 施季森[1] 

机构地区：[1]南京林业大学,林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2013年第6期153-156,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏省农业科技自主创新资金项目(CX(12)2018);国家林业局"948"项目(2013-4-34)

摘　　要：采用涂片法和45S rDNA荧光原位杂交技术,对野百合(Lilium brownii)和金佛山百合(L.jinftishanense)进行了核型分析和45S rDNA在其染色体上的物理定位。核型分析结果表明:野百合的核型为3B,金佛山百合核型为3A;野百合的核型公式为2n=2x=24=4m+8st(4SAT)+12t,金佛山百合的核型公式为2n=2x=24=4m+8st+12t;野百合和金佛山百合的核不对称系数分别为82.12%和82.65%。荧光原位杂交结果表明:野百合有6个45S rDNA位点,它们分别位于3、6、9号染色体上;金佛山百合有8个45S rDNA位点,它们分别位于1、3、6、8号染色体上。The karyotype analysis and physical localization of 45S rDNA on the chromosomes of Lilium brownii and L. jinfiishanense were ＂studied by using the smear methods and the technology of 45S rDNA fluorescence in situ hybridiza- tion. The result of karyotype analysis indicated that the karyotype of L. brownii was 3 B, the karyotype of L. jinfiishanense was 3A; The karyotype formula was 2n =2x =24 =4m ＋ 8st（4SAT） ＋ 12t for L. brownii, 2n =2x =24 =4m ＋8st ＋ 12t for L. finfiishanense;As, k was 82.12% and 82.65% respectively. The result of fluorescence in situ hybridization showed that L. brownii had 6 loci of 45S rDNA distributed on chromosome No. 3, No. 6 and No. 9 and L. finfiishanense had 8 on No. 1, No. 3, No. 6 and No. 8, respectively.

关 键 词：野百合 金佛山百合 核型分析 荧光原位杂交 

分 类 号：Q948[生物学—植物学]