杉木成年优树组培生根研究  

Studies on factors influencing rooting efficiency from mature Chinese fir superior clones in vitro

作　　者：周小红[1] 张元莉[1] 李美平[1] 赵芳芳[1] 陈金慧[1] 郑仁华[2] 肖晖[2] 黄金华 张志才 李勇 郑雪燕 施季森[1] 

机构地区：[1]南京林业大学,林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037 [2]福建省林业科学研究院,福建福州350000 [3]福建省洋口国有林场,福建顺昌353211

出　　处：《南京林业大学学报（自然科学版）》2013年第6期169-172,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家林业公益性行业专项项目(201004049);福建省林木种苗科技攻关项目(闽林科【2009】4号);国家自然科学基金重点项目(30930077);江苏高校优势学科建设工程资助项目(PAPD);江苏省高校研究生科研创新计划资助项目(CXZZ11_0533)

摘　　要：以3个成年杉木优良无性系茎段为外植体，研究基因型、生长调节剂、活性炭、基本培养基对杉木生根效率的影响。结果表明：基因型和生长素NAA是影响杉木生根效率的主要因素。1#基因型的杉木生根能力强，在1／4DCR培养基（添加IBA1．0mg／L或NAA0．5～1．0mg／L）上生根率可达90％以上；2#基因型的杉木生根能力较弱，添加1．5mg／LIBA或1．0mg／LNAA的1／2DCR培养基更适合其生根；对于3#基因型的杉木，3／4DCR培养基最适合其生根，添加IBA1．5mg／L或NAAl．0mg／L时生根率最高。对3个基因型的生根试验研究表明，活性炭对杉木生根不能起到促进作用。Using three different genotypes of shoot sections of mature superior clones of Cunninghamia lanceolata, the effect of factors such as plant growth regulators （PGRs）, genotypes, basal medium and activated charcoal were studied for rooting frequency. The results showed that genotypes and NAA were key factors influencing rooting efficiency. Geno- type 1# was more capable of rooting. The basal medium 1/4 DCR added with IBA 1.0 mg/L or NAA 0.5 - 1.0 mg/L could increase rooting efficiency to the level of 90%. Genotype 2# had a weaker ability of rooting. The 1/2DCR basal medium added with IBA 1.5 mg/L or NAA 1.0 mg/L was better in adventitious＇roots induction for this genotype. For genotype 3#, it had the best rooting efficiency when the level of IBA was 1.5 mg/L or the level of NAA was 1.0 mg/L, 3/4 DCR was fit for rooting. Addition of activated charcoal could not effectively improve rooting in this experiment.

关 键 词：生根能力 杉木 成年 基本培养基 组培 优树 基因型 优良无性系 

分 类 号：Q813.12[生物学—生物工程]