黄瓜细菌性萎蔫病菌分子诊断研究  

Study on molecular diagnosis of cucumber bacterial wilt

作　　者：王楠[1,2] 张改红[1] 王伟[2] 

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450001 [2]华东理工大学生物工程学院/生物反应器工程国家重点实验室,上海200237

出　　处：《郑州轻工业学院学报（自然科学版）》2013年第5期21-25,共5页Journal of Zhengzhou University of Light Industry:Natural Science

基　　金：国家自然科学基金项目(30871664);国家重点实验室专项经费(2060204)

摘　　要：从田间黄瓜细菌性萎蔫病植株分离获得病原菌,比对其近源种的16S rDNA序列,确定该病原菌为嗜维管束欧文氏菌(Erwinia tracheiphila),并设计出特异引物ET-P1/ET-P2.经过对该引物的PCR扩增条件优化,扩增出一条794 bp的黄瓜细菌性萎蔫病菌特异条带,检测灵敏度达100 fg/μL,可准确扩增出黄瓜发病植株和根围土壤中的DNA片段.The pathogen was obtained by separating the cucumber bacterial wilt plant,and by comparing with the closely-related species 16Sr DNA sequence,it was identified as Erwinia tracheiphila and specific primers ET-P1 / ET-P2 was designed. A 794 bp cucumber bacterial wilt special band can be amplified by optimizing the PCR amplification of ET-P1 / ET-P2 primers with the detection sensitivity of 100 fg / μL,and can amplify the DNA of bacterial wilt cucumber and rhizosphere soil. The molecular diagnosis technology of cucumber bacterial wilt.

关 键 词：黄瓜细菌性萎蔫病 嗜维管束欧文氏菌 特异性引物 分子诊断技术 

分 类 号：TS255.2[轻工技术与工程—农产品加工及贮藏工程] S432.42[轻工技术与工程—食品科学与工程]