白芍总苷对角质形成细胞增生及ICAM-1表达的影响  

Effects of total glucosides of paeony on proliferation of human HaCaT keratinocytes and expression of ICAM-1

作　　者：王佳媚[1] 杨桂兰[2] 赵敏[2] 王剑峰[2] 潘之[2] 肖辉[2] 

机构地区：[1]兰州大学第二临床医学院,兰州730000 [2]兰州军区兰州总医院皮肤科,甘肃省干细胞与基因药物重点实验室

出　　处：《实用皮肤病学杂志》2013年第6期331-334,共4页Journal of Practical Dermatology

基　　金：中华医学会皮肤性病学分会-朗生医药皮肤病学研究基金

摘　　要：目的探讨白芍总苷(TGP)对人永生化角质形成细胞(HaCaT细胞)增生及分泌细胞间黏附分子(ICAM)-1的影响,探讨可能涉及的信号传导通路。方法用500 U/ml干扰素(IFN)-γ诱导HaCaT细胞,不同浓度TGP(0.5～312.5μg/ml)作用于HaCaT细胞。四甲基偶氮唑盐(MTT)法观察TGP对HaCaT细胞增生活性的影响。酶联免疫吸附试验(ELISA)及荧光免疫组化法检测HaCaT细胞表达ICAM-1的水平。结果 TGP在低浓度(0.5～25.0μg/ml)时对HaCaT细胞增生活性有促进作用,在高浓度(62.5～125.0μg/ml)时对HaCaT细胞增生活性呈抑制作用。0.5～25.0μg/ml TGP可显著降低HaCaT细胞表达ICAM-1的水平(P<0.05,P<0.01)。结论 TGP对IFN-γ上调HaCaT细胞分泌ICAM-1有显著的抑制作用,可能是TGP抗炎作用机制之一。Objective To evaluate the effects of total glucosides of paeony （TGP） on cell proliferation of human HaCaT keratinocytes and the expression of ICAM-1, and explore the signaling pathways which may be involved in the process. Methods HaCaT cells were stimulated by 500 U/mL IFN-γ, then incubated with TGP of various concentrations （0.5 to 312.5 μg/ml）. MTT assay was performed to detect the cell proliferation of HaCaT cells. The expression of ICAM-1 in HaCaT cells was evaluated by enzyme linked immunosorbent assay （ELISA） and lfuorescent immunohistochemical technology. Results The proliferation of HaCaT cells was promoted by TGP of low concentrations （0.5 to 25.0μg/ml）, but inhibited by TGP of high concentration （62.5 to 125.0μg/ml）. TGP of low concentrations （0.5 to 25.0μg/mL） signiifcantly reduced the expression of ICAM-1. Conclusion TGP signiifcantly inhibited the upregulated ICAM-1 secretion of HaCaT cells which induced by IFN-γ, that may be one of the anti-inlfammatory mechanism of TGP.

关 键 词：白芍总苷 人永生化角质形成细胞 细胞间黏附分子-1 Γ干扰素 

分 类 号：R285.5[医药卫生—中药学] R364.31[医药卫生—中医学]