山东玉米粗缩病病原S10序列分析  

Sequence Analysis on S10 of Pathogens Isolated from Maize Plants with Rough Dwarf Disease in Shandong

作　　者：王升吉[1] 张恒木[2] 赵玖华[1] 吴为奇[2] 赵亚[1] 羊健[2] 尚佑芬[1] 袁园园 

机构地区：[1]山东省农业科学院植物保护研究所/山东省植物病毒学重点实验室,济南250100 [2]浙江省农业科学院病毒与生物技术研究所/植物有害生物防控国家重点实验室培育基地,杭州310021 [3]济南市农业科学院,济南250316

出　　处：《玉米科学》2013年第6期26-30,共5页Journal of Maize Sciences

基　　金：国家公益性行业(农业)科研专项子课题(201003031);科技部-浙江省共建国家重点实验室基金项目(2010DS700124-KF1106);山东省科技发展计划项目(2009GG10009015);济南市科技发展计划和高校院所自主创新计划项目(200906024-2)

摘　　要：利用采自山东章丘、济宁表现典型粗缩病症状的玉米植株样品进行ELISA和RT-PCR检测。间接ELISA结果表明,二者均与水稻黑条矮缩病毒(RBSDV)多克隆抗体表现阳性反应。RT-PCR扩增出该病毒基因组S10约1.8 kb片段。获得的1 801 bp全长序列与已发表的相关序列进行同源性分析和系统进化树分析,结果表明,章丘分离物克隆JNZ-14序列、济宁分离物克隆JN-8序列均属于RBSDV,但其与玉米粗缩病毒(MRDV)的进化关系比其与南方水稻黑条矮缩病毒(SRBSDV)的关系还要近,且2个分离物分别处于属于RBSDV的Ⅱ或Ⅰ两大分支中。Typical rough dwarf symptom from Zhangqiu and Jining of Shandong was detected by ELISA and RT-PCR methods. The results from indirect ELISA indicated that, both the samples showed positive reaction with RBSDV polyclonal antibody. RT-PCR amplified about 1.8 kb segment of the viral genome S10. the 1.8 kb full-length sequence acquired had made homology and phylogenetic tree analysis with published sequences related. The results showed that, the Zhangqiu isolate clones JNZ-14 sequence, the Jining isolate clones, JN-8, belongs to Rice black-streaked dwarf virus（RBSDV）,but the evolutionary relationship with MRDV were even closer than theirs with southern rice black-streaked dwarf virus（SRBSDV）, moreover, the two isolates were in largeⅡorⅠbranches of RBS-DV respectively.

关 键 词：玉米 粗缩病 序列 

分 类 号：S513.034[农业科学—作物学]