调脂颗粒对HepG2细胞SR-BI/CLA-1的调控研究  

Regulation of Tiaozhi Granules on SR-BI /CLA-1 in HepG2 cells

作　　者：陈竞纬[1] 郁晓[2] 董丽霞[3,4] 钱培刚[3,4] 赵笑东[1] 顾振纶[3,4] 

机构地区：[1]苏州市中医医院,江苏苏州215009 [2]苏州大学生物医学研究院,江苏苏州215123 [3]苏州大学药学院药理学系,江苏苏州215123 [4]苏州中药研究所,江苏苏州215123

出　　处：《中成药》2013年第12期2560-2564,共5页Chinese Traditional Patent Medicine

基　　金：江苏省苏州市科学技术管理局应用基础研究计划(SYS201005;SYS201052);国家中医药管理局学术流派传承工作室建设项目(2012年)

摘　　要：目的研究调脂颗粒(姜黄、蒲黄、泽泻)对HepG2细胞SR-BI/CLA-1启动子活性、mRNA和蛋白表达的影响。方法 SD大鼠予调脂颗粒和立普妥喂养3 d后采血获得含药血清,HepG2细胞随机分为5组并予以相应的含药血清处理。共转染SR-B1/CLA-1报告质粒及pRL-SV40,荧光素报告基因法检测启动子活性。SYBR Green I实时荧光定量PCR和Western blot方法检测SR-BI/CLA-1 mRNA和蛋白的表达。结果调脂颗粒上调了SR-BI/CLA-1启动子活性、mRNA和蛋白质的表达(P<0.05或P<0.01),且呈剂量依赖性趋势。结论调脂颗粒能上调SR-BI/CLA-1的表达,这可能是其调节血脂的作用机制之一。AIM To clarify the mechanism of Tiaozhi Granules （ Cureumae longae Rhizoma, Typhae Pollen, Alismatis Rhizoma） for regulating blood lipid, the promoter activity, mRNA and protein expression of SR-B1/CLA- 1 in HepG2 cells. METHODS SD rats were fed with Tiaozhi Granules or Liptor for three days. Then the serums contained different drugs were separated. HepG2 cells were assigned into five groups randomly, following stimula- tion of the different drug-contained serum. Co-transfeeted SR-B1/CLA-1 and RL-SV40 promoters into HepG2 cells, then promoter aetivity was measured by Lueyferase reporter gene assay. The mRNA and protein expressions of SR-B1/CLA-1 were examined by real-time PCR and Western Blot respectively. RESULTS The promoter activ- ity, mRNA and protein of the SR-B1/CLA-1 were up-regulated significantly by Tiaozhi Granules （P 〈0. 05 or P 〈 0. 01 ）. CONCLUSION Tiaozhi Granules can up-regulate SR-B1/CLA-1 gene expression resulting in total blood lipid modulation in vivo.

关 键 词：调脂颗粒 HEPG2 SR-BI CLA一1 

分 类 号：R285.5[医药卫生—中药学]