莪术油诱导卵巢癌顺铂耐药细胞凋亡及对STAT信号传导通路的影响  

Induction of apoptosis and influence on STAT pathway in human cisplatin-resistantovary cancer cell line by Zedoary Oil

作　　者：李荣霞[1] 刘首娟 孔德胜[1] 李敏[1] 李艳芳[1] 史淑红[1] 

机构地区：[1]河北医科大学第二医院,河北石家庄050000

出　　处：《中成药》2013年第12期2570-2576,共7页Chinese Traditional Patent Medicine

摘　　要：目的探讨莪术油对人卵巢癌顺铂化疗耐药细胞SKOV3/DDP生长抑制作用及其对STAT信号传导通路的影响。方法在培养SKOV3/DDP细胞株中加入不同质量浓度的莪术油进行刺激,利用光学显微镜观察细胞形态学变化,MTT;免疫组化及半定量逆转录-聚合酶链反应(RT-PCR)技术检测STAT3、Survivin、Caspase-3的表达情况。结果显微镜下可见不同质量浓度莪术油作用后细胞数量,形态学有明显改变;莪术油对SKOV3/DDP细胞增殖有抑制作用,呈明显的时间-剂量关系(P<0.05);莪术油及其联合顺铂作用后,STAT3、Survivin表达下调,Caspase-3表达上调。结论莪术油抑制人卵巢癌耐药细胞株增殖并诱导其凋亡,其机制可能与下调STAT3,Survivin,上调Caspase-3表达有关。AIM To investigate the inhibitory effect of Zedoary Oil on cisp latin-resistant ovary cancer cell line SKOV3/DDP and expression of the STAT3 pathway. METHOD The cellular morphology was observed by adding different concentrations of Zedoary Oil into the proliferation of SKOV3/DDP cells by light microscopy and MTF. The protein expression of STAT3, Survivin, and Caspase-3 was detected by immunohistochemistry and RT-PCR. RESULTS The number of SKOV3/DDP cells was significantly reduced, and cellular morphology was also obvi- ously changed by light microscope. The proliferation of SKOV3/DDP cells was markedly inhibited by Zedoary Oil in a dose-dependent and time-dependent manner （ P 〈 0. 05 ）. The protein expression of Caspase-3 was activated, the expressional levels of STAT3 and survivin proteins decreased significantly under the influence of Zedoary Oil and the combination with cisplatin. CONCLUSION Zedoary Oil can inhibit the proliferation of human eisplatin-re- sistant ovary cancer SKOV3/DDP cell line in vitro, and induce apoptosis in relating to the down regulation of STAT3 and Survivin, and up-regulating Caspase-3 protein.

关 键 词：莪术油 卵巢癌 顺铂耐药细胞 信号传导子与转录激活子3 生存素 

分 类 号：R285.5[医药卫生—中药学]