齐墩果酸和绿原酸对HepG2细胞及P450酶的影响  

Effects of oleanolic acid and chlorogenic acid on HepG2 cells and P450 enzyme expression

作　　者：汤浩[1,2] 刘晓莉[2] 王锐[2] 高庆剑 陆铖[2] 孙志博[3] 

机构地区：[1]甘肃省人民医院药剂科,甘肃兰州730000 [2]宁夏医科大学药学院药理系,宁夏银川750000 [3]兰州大学基础医学院遗传所,甘肃兰州730000

出　　处：《中成药》2013年第12期2576-2580,共5页Chinese Traditional Patent Medicine

基　　金：甘肃省技术研究与开发专项计划(1105TCYA024);兰州市科技计划项目(2010-1-72)

摘　　要：目的观察齐墩果酸和绿原酸对HepG2细胞增殖、周期变化及CYP相关基因表达的影响。方法以利福平为对照,采用四甲基偶氮唑盐(MTT)比色法、流式细胞术、实时定量PCR技术检测,测定不同质量浓度齐墩果酸、绿原酸的作用。结果齐墩果酸、绿原酸对HepG2细胞增殖有明显抑制作用,阻滞HepG2细胞停留于S期。齐墩果酸和利福平均能诱导CYP1A1和CYP3A4表达。绿原酸抑制CYP1A1和CYP3A4表达。结论齐墩果酸、绿原酸均能影响细胞周期来抑制HepG2细胞生长,并显示不同质量浓度对CYP1A1、CYP3A4 mRNA的表达影响不一致。AIM To investigate the effects of oleanolic acid and chlorogenic acid on proliferation and cell cycle of HepG2 cells and its relative CYP450 genes expression. METHODS Rifampicin as the control drug, the MTY method, Flow cytometry, and Real time quantitative PCR technique were used in this study. RESULTS Chloro- genie acid and oleanolic acid could inhibit cell proliferation of HepG2 cells, and arrest cell cycle at S stage. Olean- olic acid induced CYP1A1, CYP3A4 mRNA , but chlorogenic acid inhibited CYP1A1. CONCLUSION Olean- olic acid and chlorogenic acid can inhibit the proliferation of HepG2 cells, but show inconsistent effects on drug metabolic enzyme CYP1A1, CYP3A4 mRNA expression.

关 键 词：齐墩果酸 绿原酸 HEPG2细胞系 细胞增殖 细胞周期 细胞色素P450酶 

分 类 号：R285.5[医药卫生—中药学]