蒲黄-五灵脂配伍前后化学成分溶出变化的定量分析  

Analysis of the changes of chemical components dissolution of Puhuang-Wulingzhi before and after compatibility

作　　者：薛萍[1,2] 欧阳臻[1] 宿树兰[2] 周卫[2] 段金廒[2] 白永亮[1,2] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]南京中医药大学江苏省方剂高技术研究重点实验室,江苏南京210046

出　　处：《中成药》2013年第12期2689-2693,共5页Chinese Traditional Patent Medicine

基　　金：江苏省优势学科建设工程中医学一级学科建设项目(YS2012ZYX314);江苏省高校自然科学重大基础研究项目(06KJA36022;11KJA360002);江苏省方剂高技术研究重点实验室建设项目资助(BM2010576)

摘　　要：目的评价蒲黄-五灵脂配伍前后有机酸、黄酮类成分的溶出变化,以探讨药对配伍的科学性与合理性。方法采用HPLC-PDA方法同时分析测定样品中没食子酸、原儿茶酸、香草酸、对香豆酸、香蒲新苷和异鼠李素-3-O-新橙皮糖苷的量,采用Diamonsil C18色谱柱,梯度洗脱的方法进行分析测定。结果蒲黄、五灵脂配伍后香蒲新苷、异鼠李素-3-O-新橙皮糖苷溶出增加,均高于蒲黄、五灵脂单煎液和单煎混合液;没食子酸、原儿茶酸、香草酸、对香豆酸4个有机酸成分经配伍后溶出量低于单煎液,但高于单煎混合液。结论本实验建立了同时测定蒲黄-五灵脂中有机酸和黄酮类成分的HPLC-PDA方法,为揭示其效应物质基础提供科学依据与参考。AIM To evaluate the influence of compatibility of Puhuang （ Typhae Pollen） and Wulingzhi （ Tro- gopterori Faeces） on the changes in dissolution of active substances. METHODS Four organic acids and two fla- vonoids were analyzed simultaneously by HPLC-PDA. A Diamonsil Cls column （ 250 mm x 4.6 ram, 5 pm ） was used with methanol and 0. 5% acetic acid solution as mobile phase in gradient elution manner. RESULTS This method showed a good linearity for six components in their respective range and the average recoveries fell between 95.54% and 105.35% , with RSD within 1.78% - 3.07%. As a result, the contents of typhaneoside and isoham- netin-3-O-neohesperidoside increased after compatibility while the contents of gallic acid, protocatechuic acid, va- nillic acid, and p-coumaric acid, decreased. CONCLUSION The method for simultaneous determination of or- ganic acids and flavonoids in compatibility of Typhae Pollen and Trogopterori Faeces can be used in claritying the bioactive components research.

关 键 词：蒲黄 五灵脂 化学成分 HPLC-PDA 

分 类 号：R284.1[医药卫生—中药学]