结核患者CD19^+ CD5^+ CD1d^+ B细胞表达及其对Th17细胞的影响  

Association between Th17 response and CD19^+ CD1d^+ CD5^+ B cells in patients with tuberculosis

作　　者：李关忠[1] 廖明凤[1] 曾光明[1] 张影[1] 张明霞[1] 陈心春[1] 邓群益[1] 

机构地区：[1]广东省深圳市第三人民医院,深圳市结核病临床重点专科,518112

出　　处：《实用医学杂志》2013年第23期3805-3807,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金面上项目(编号:81241065);深圳市科技计划项目(编号:20120264)

摘　　要：目的:分析结核患者Th17应答与CD19+CD5+CD1d+B细胞频率的相关性。方法:应用流式细胞术检测健康对照、结核潜伏感染者、结核患者外周血中Th17应答水平及CD19+CD5+CD1d+B细胞频率。结果:结核患者Th17应答水平为(3.34±1.35)%,显著低于健康对照(4.60±1.56)%(q=6.56,P<0.001)和结核菌潜伏感染者(4.13±1.91)%(q=2.96,P<0.05);结核患者CD19+CD5+CD1d+B细胞频率为(28.29±11.03)%,显著高于健康对照(21.05±10.86)%(q=3.69,P<0.05)和潜伏感染者(20.46±9.88)%(q=3.59,P<0.05);结核患者Th17应答与CD19+CD5+CD1d+B细胞频率呈负相关性(r=-0.467 3,P<0.05)。结论:活动性结核病患者Th17细胞应答受抑制,这种抑制可能与CD19+CD5+CD1d+B细胞频率的增多有关。Objective To investigate the correlation between the frequencies of Thl7 cells and CD19 CDldCD5 B cells in peripheral blood of tuberculosis （TB） patients. Methods The frequencies of Thl7 cells and CD19CDldCD5 B cells in the healthy controls,latent tuberculosis infection and the TB patients were detected by flow cytometry assay. Results The frequency of Thl7 cells in patients with active tuberculosis was （3.34 ＋ 1.35）% ,which was significantly lower than that in the healthy donors （HD） （4.60 _＋ 1.56%） （q = 6.56,P 〈 0.001） and than that in individuals with latent tuberculosis infection（LTBI） （4.13 1.91）%（q = 2.96,P 〈 0.05）, respectively. The proportion of CD19＋CDldCD5B cells in TB patients was （28.29 _＋ 11.03）%,which was much higher than that in HDs（21.05 10.86）%（q = 3.69,P 〈 0.05） and than that in LTBIs（20.46 9.88）%（q = 3.59,P 〈 0.05）; Increasing CD19 CDldCD5 B cells in TB patients inversely correlated with Thl7 response in patients with TB. Conclusion Thl7 response in patients with tuberculosis was suppressived and the suppressive Thl7 response in patients with tuberculosis may be associated with increasing CD19CDldCD5 B cells.

关 键 词：结核 Thl7应答 CDl9+CD5+CDld+B细胞 

分 类 号：R181.3[医药卫生—流行病学]