环介导等温扩增对肺结核快速诊断的分析研究  

Analysis of loop-mediated isothermal amplification for the rapid diagnosis of pulmonary tuberculosis

作　　者：江勇 钱明 陈涛 李海成 陈燕梅 卓文基 郭卉欣 钟球 周琳 

机构地区：[1]广东省结核病控制中心参比实验室,广州市510630

出　　处：《实用医学杂志》2013年第23期3818-3820,共3页The Journal of Practical Medicine

基　　金：广东省自然科学基金项目(编号:WSTJJ20120319130 623198210011211);国家科技重大专项:艾滋病和病毒性肝炎等重大传染病防治:深圳市宝安区广州市番禺区结核病防治示范区(编号:2012ZX10004903)

摘　　要：目的:探讨环介导等温扩增检测方法在肺结核诊断中的价值。方法:选取408份初诊疑似肺结核患者的痰标本,同时进行直接涂片抗酸染色法、改良罗氏培养基培养法和环介导等温扩增法检测。结果:在检测的408份痰标本,直接涂片抗酸染色法检出阳性标本86份,阳性率21.1%(86/408);培养检出阳性标本112份,阳性率27.5%(112/408);LAMP检出阳性标本110份,阳性率27.0%(110/408)。以改良罗氏培养基培养法检测结果为标准,涂片法的灵敏度为72.3%(81/112),特异性为98.3%;LAMP灵敏度为92.9%(104/112),特异性为98.0%。检测结果具有统计学差异(χ2=16.42,P<0.05)。结论:LAMP在疑似肺结核患者的诊断中具有较高的灵敏度和特异性,可作为结核病诊断和鉴定的重要依据。Objective To investigate the clinical diagnostic value of loop-mediated isothermal amplification （LAMP） for mycobacterium tuberculosis. Methods Four hundred and eight cases of sputum samples from patients with suspected pulmonary tuberculosis were collected, and then were detected by LAMP method, direct smear acid- fast attaining and L-J medium. Results In 408 cases of sputum samples, 86 cases were smear positive （21.1%）, 112 cases were culture positive （27.5%） and ll0 samples were Lamp positive （27.0%）. With the L-J medium as the standard, the sensitivity and specificity of smear and LAMP were 72.3%, 98.3% and 92.9%, 98.0%, respectively. Conclusion The LAMP method had high sensitivity and specificity in those samples with suspected pulmonary tuberculosis, and could give an important advice for treatment for pulmonary tuberculosis.

关 键 词：结核.肺 环介导等温扩增法 诊断 

分 类 号：R521[医药卫生—内科学]