景天属植物叶绿体DNA与核DNA分步提取方法研究  

Step-by-step Extraction Method of Chloroplasts DNA and Nuclear DNA of Sedum Plants

作　　者：郝海叶 张洋[1] 那冬晨[1] 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《安徽农业科学》2013年第25期10230-10231,共2页Journal of Anhui Agricultural Sciences

基　　金：山西省大学生创新性实验项目(105075)

摘　　要：[目的]研究景天属植物叶绿体DNA与核DNA分步提取方法。[方法]以景天属植物佛甲草、八宝景天、华北景天、费菜和垂盆草为研究材料,进行叶绿体DNA和核DNA的分步提取,通过琼脂糖凝胶电泳和PCR扩增进行检测。[结果]改进后的方法提取的核DNA和叶绿体DNA的质量好,纯度高。以叶绿体DNA和核DNA为模板进行PCR扩增均得到理想的条带,扩增率为100%,重复性好。[结论]该研究为叶绿素DNA和核DNA的提取提供了新的思路。[Objective] To study step-by-step extraction method of chloroplasts DNA and nuclear DNA of Sedum plants.[Method] With Sedum lineare Thunb,Sedum spectabile Boreau,Sedum tatarinowii Maxim,Sedum aizoon L.and Sedum sarmentosum Bunge as study material,step-bystep extraction of chloroplasts DNA and nuclear DNA were conducted.DNA was tested by agarose gelelectrophoresis and polymerase chain reaction.[Result] The results showed that better quality and higher purity chloroplasts DNA and nuclear DNA were obtained by improved method.The results of polymerase chain reaction from chloroplasts DNA and nuclear DNA templates were desirable,amplification rate of 100％ and highly repeatable.[Conclusion] The study provides a new thought for extraction of chloroplasts DNA and nuclear DNA.

关 键 词：景天属植物 叶绿体DNA 核DNA 分步提取 

分 类 号：S188[农业科学—农业基础科学]