DRD_3基因敲除鼠饲养繁殖及基因型鉴定  

Breeding Reproduction of DRD_3 Gene Knockout Mice and Its Genotype Identification

作　　者：张宝[1] 魏曙光[1] 张洪波[1] 余兵[1] 冯祖飞[1] 官方霖 徐月红[1] 高春燕[1] 李生斌[1] 

机构地区：[1]西安交通大学医学院卫生部法医学重点实验室,环境与疾病相关基因教育部重点实验室,陕西西安710061

出　　处：《安徽农业科学》2013年第26期10657-10658,共2页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31301949);中国博士后科学基金资助项目(2013M532056);陕西省博士后科学基金资助项目

摘　　要：[目的]对于DRD3基因敲除小鼠的饲养繁殖以及鉴定方法进行分析,为进一步研究多巴胺及其受体的功能提供动物模型。[方法]引进的DRD3基因敲除小鼠均为杂合子基因型,1雌1雄同窝进行饲养繁殖,从仔鼠中提取基因组DNA,进行PCR鉴定。[结果]成功获得的子代小鼠有敲除杂合子、野生型及敲除纯合子3种基因型。DRD3敲除杂合子基因型小鼠的饲养繁殖获得成功,亦获得较多敲除纯合子基因型小鼠。[结论]正确的饲养繁殖管理及基因型鉴定方法可从DRD3基因杂合子小鼠成功获得纯合子小鼠。[Objective] To discuss the breeding and identification methods of DRD3 gene knockout mice and lay the foundation for further study on the function of dopamine and its receptors.[Method] Heterozygote mice were bred and reproduced.Genome DNA extracted from the mice＇ s tails were amplified by using PCR method for genotype identification.There were three genotypes （heterozygote,wild-type and homozygote） in the offspring.[Result] The experimental model for DRD3 gene knockout mice was successfully established and more DRD3 knockout mice were reproduced.[Conclusion] Appropriate methods of breeding,reproducing,and identifying are effective ways to gain DRD3 gene knock-out mice from heterozygote mice.

关 键 词：DRD3基因 基因敲除 鉴定 

分 类 号：S813[农业科学—畜牧学]