切口痛大鼠脊髓Toll样受体4及其下游细胞因子mRNA表达的变化  

Changes in expression of Toll-like receptor 4 mRNA and its down-stream cytokines mRNA in spinal cord in a rat model of incisional pain

作　　者：李治松[1] 李艳娜[1] 杜英英[1] 李会新[1] 张卫[1] 阚全程 

机构地区：[1]郑州大学第一附属医院麻醉科,450052 [2]河南省高等学校临床医学重点学科开放实验室

出　　处：《中华麻醉学杂志》2013年第10期1223-1225,共3页Chinese Journal of Anesthesiology

基　　金：河南省医学科技攻关项目（201203037）

摘　　要：目的 切口痛大鼠脊髓Toll样受体4（TLR4）及其下游细胞因子mRNA表达的变化.方法 健康成年雄性SD大鼠58只,体重180 ～ 220 g,建立大鼠切口痛模型.于术前、术后0.5、1、2、8、12h、1、2、3、5和7d时测定术侧与非术侧机械缩足反应阈（MWT）;于术前、术后2、8h、1、2、3、5和7d时采用荧光定量PCR法检测脊髓TLR4、IL-1β和TNF-α的mRNA的表达.结果 与术前比较,大鼠术侧术后0.5h～5d MWT降低,术后2、8h、1、2和3d时脊髓TLR4 mRNA、IL-1 mRNA、TNF-α mRNA表达上调（P＜0.05）,非术侧MWT差异无统计学意义（P＞0.05）;术侧MWT术后2h时最低,之后逐渐升高,脊髓TLR4 mRNA术后1d时表达水平最高,IL-1 mRNA、TNF-α mRNA术后8h时表达水平最高（P＜0.05）.TLR4 mRNA表达水平与术侧MWT呈负相关（相关系数-0.484,P＜0.05）,IL-1β mRNA和TNF-α mRNA表达水平与TLR4 mRNA表达水平呈正相关（相关系数分别0.294、0.540,P＜0.05）.结论 切口痛大鼠脊髓TLR4及下游炎性因子IL-1β、TNF-α的mRNA表达上调,该变化可能参与了切口痛的维持,但并未起主要作用.Objective To evaluate the changes in the expression of Toll-like receptor 4 （TLR4） mRNA and its down-stream cytokines IL-1β and TNF-α mRNA in spinal cord in a rat model of incisional pain.Methods Fifty-eight male Sprague-Dawley rats,weighing 180-220 g,were used in this study.A 1-cm longitudinal incision was made through skin,fascia and muscle of the plantar aspect of the hindpaw in isoflurane-anesthetized rats.Mechanical paw withdrawal threshold to yon Frey filament stimulation （MWT） on the operated and non-operated sides was measured before operation and at 0.5,1,2,6 and 12 h and 1,2,3,5 and 7 days after operation.Six rats were chosen and sacrificed before operation and at 2 and 8 h and 1,2,3,5 and 7 days after operation.Their lumbar segments （L4-6） of the spinal cord were removed for determination of the expression of TLR4,IL-1β and TNF-α mRNA by real-time PCR.Results Compared with the baseline value before operation,MWT on the operated side was significantly decreased at 0.5 h-5 days after operation,and the expression of TLR4,IL-1β and TNFα mRNA was up-regulated at 2 and 8 h and 1,2 and 3 days after operation （P ＜ 0.05）,and no significant change was found in MWT on the non-operated side （P ＞ 0.05）.MWT on the operated side was lowest at 2 h after operation and then gradually increased,the expression of TLR4 mRNA peaked on 1 day after operation,and the expression of IL-1 and TNF-α mRNA peaked at 8 h after operation （P ＜ 0.05）.The TLR4 mRNA expression was negatively correlated with MWT on the operative side （r =-0.484,P ＜ 0.05）,and IL-1 mRNA and TNF-α mRNA expression was positively correlated with TLR4 mRNA （r =0.294 and 0.540,respectively,P ＜ 0.05）.Conclusion The expression of TLR4 mRNA and its down-stream cytokines IL-1β and TNF-α（mRNA in spinal cord is up-regulated,this change is involved in the maintenace of incisional pain,but it does not play an important role.

关 键 词：TOLL样受体4 白细胞介素1Β 肿瘤坏死因子Α RNA 信使 脊髓 疼痛 手术后 

分 类 号：R614[医药卫生—麻醉学]