虎杖苷对严重烫伤大鼠心肌细胞钙信号调控的作用  

Polydatin modulates calcium signals in thermally injured cardiomyocytes

作　　者：邓建新[1] 蓝莉芹 张翠翠[1] 丁文文[1] 薛翔[1] 刘文娟[2] 

机构地区：[1]南方医科大学病理生理学教研室,广东省医学休克微循环重点实验室,广东广州510515 [2]深圳大学医学院病理生理学教研室,广东深圳518060

出　　处：《心脏杂志》2013年第6期639-643,共5页Chinese Heart Journal

基　　金：国家自然科学基金项目资助(81200122);深圳大学自然科学基金项目资助(00035904)

摘　　要：目的:观察虎杖苷(polydatin,PD)对烫伤大鼠心功能损害的钙信号调控,并探讨其对烫伤大鼠心肌细胞保护作用的机制。方法:28只成年SD大鼠随机分为4组,即假手术组、烫伤组、PD组及烫伤+PD组,每组7只大鼠(n=7)。各实验组使用Power lab系统进行实时心功能监测。手术后5 min通过静脉给予PD(10 mg/kg),12 h后分离心室肌细胞,通过激光共聚焦显微镜观测PD对烫伤大鼠心室肌细胞钙火花发放频率、钙库水平、钙瞬变的作用。结果:与烫伤组比较,烫伤+PD组大鼠左室收缩压、左心室内压力上升的最大速率(±dP/dt max)均显著增加、钙瞬变和心肌收缩力明显增强、使烫伤引起的钙库容量下降基本恢复到正常水平,烫伤引起的高频钙火花发放显著抑制(均P<0.01),钙火花时程的增加也明显抑制(P<0.05)。结论:PD可以显著抑制烫伤引起的心肌细胞肌浆网钙漏流,恢复肌浆网钙库容量水平,增强收缩期钙瞬变和心肌收缩力,从而改善烫伤引起的心功能障碍。AIM：To investigate the protective effect of polydatin （PD） on calcium signals in thermally injured cardiomyocytes. METHODS： Twenty eight adult Sprague Dawley rats were randomly divided into four groups （seven rats/group）： shamoperation group, thermal injury group, PD group and thermal injury＋PD group. Polydatin （10 mg/kg） was given intravenously 5 min after operation. Twelve hours after thermal injury, single myocytes were isolated from ventricular free wall enzymatically and loaded with Ca2＋ indicator fluo4 AM. Ca2＋ sparks and transients were recorded. RESULTS： PD significantly improved left ventricular systolic pressure （LVSP）, ＋dP/dtmax and dP/dtmax of thermal injury cardiomyocytes （P〈0.01）. Polydatin significantly increased thermal injury systolic Ca2＋ transients by about 26% and myocyte contractility （P〈0.01）, restored sarcoplasmic reticulum Ca2＋ content （P〈0.01）, inhibited thermal injuryinduced high frequency and the duration of calcium sparks （P〈0.05）. CONCLUSION： Polydatin has the capability of preventing SR leak and restoring intracellular Ca2＋ homeostasis in thermal traumatized hearts and ameliorates thermally induced cardiac dysfunction, thus exerting strong therapeutic effect against burngenerated cardiac dysfunction.

关 键 词：虎杖苷 烫伤 心肌细胞 钙信号 大鼠 

分 类 号：R282.71[医药卫生—中药学]