盐酸小檗碱对血管平滑肌细胞增殖的干预研究  

Berberine attenuates vascular smooth muscle cell proliferation

作　　者：伍兆锋[1] 温敏杰[1] 赖越元[1] 夏金堂[1] 蔡文松[1] 徐波[1] 李书华[2] 

机构地区：[1]广州市第一人民医院肝胆外科,广东广州510180 [2]广州医科大学病理教研室,广东广州510182

出　　处：《广州医学院学报》2013年第4期22-25,共4页Academic Journal of Guangzhou Medical College

基　　金：广东省科技计划项目(2009B060700019);广州市卫生局重点项目(201102A212018);广州市中医药和中西药结合科技项目(2009NO.6);广州市教育局科技攻关项目:08A096

摘　　要：目的：探讨盐酸小檗碱对抑制血管平滑肌细胞增殖的干预机制。方法：分离培养大鼠血管平滑肌细胞（vascularsmoothmllsclecells，VSMC），并分为实验组，对照组及空白组；MTT法选择盐酸小檗碱干预浓度；MTT法和[3H]-胸腺嘧啶核苷渗入法检测VSMC增殖率；Westernblotting方法检测ERKl／2、Akt的磷酸化蛋白的变化。结果：VSMC实验组细胞增殖率与对照组相比明显下调，P〈0．05；小檗碱干预后，VSMC细胞ERKl／2、Akt的磷酸化蛋白的表达明显下调，与对照组相比，P〈0．05。结论：盐酸小檗碱对血管平滑肌细胞的增殖有一定的抑制作用，可能与其抑制ERKl／2、Akt等的表达有关。Objective：To investigate the mechanisms regarding berberine attenuates vascular smooth muscle cell proliferation. Methods： Vascular smooth muscle cell （VSMC） in rats were Isolated, cultured and allocated to experimental group, control group and blank group. The concentration of berberine was determined by using the thiazolyl blue （MT]＇） approach, and the rate of VSMC proliferation was assayed by MTF and [ 3H ]-thymidine infiltration method. Western blotting was employed to detect ERK1/2 and Akt expression. Results： Compared with control group, the proliferation of VSMC in experimental group was suppressed by berberine （ P 〈 0.05 ）. Intervention with berberine resulted in marked down-regulated phosphorylated proteins of ERK1/2 and Akt （P 〈 0.05 ）. Conclusion ： The inhibitory effects of berberine on VSMC proliferation may be related to suppression of ERK1/2 and Akt expression.

关 键 词：盐酸小檗碱 肝移植 血管狭窄 

分 类 号：Q28[生物学—细胞生物学]