白细胞介素-17在糖尿病大鼠肾组织中的表达及意义  

Expression of Interleukin-17 in Renal Tissue of Diabetic Rats

作　　者：於文丽[1] 吴小燕[1] 李晓宁[1] 姚涛[1] 

机构地区：[1]武汉大学中南医院肾内科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2013年第6期841-844,共4页Medical Journal of Wuhan University

基　　金：湖北省自然科学基金资助项目(编号:2012FFB04306)

摘　　要：目的:观察白细胞介素-17(IL-17)在糖尿病大鼠肾组织的表达。方法:90只雄性SD大鼠随机分为对照组(Con组)、1型糖尿病组(T1DM组)及2型糖尿病组(T2DM组),链脲佐菌素(STZ,50mg/kg)制备1型糖尿病模型,高脂高糖饮食加STZ(25mg/kg)制备2型糖尿病模型。于2周、4周末用ELISA法测血、尿IL-17水平;并测尿白蛋白排泄率(UAER)、C反应蛋白(CRP)。4周末用免疫组化染色及Western印迹分析肾组织IL-17表达。结果:与对照组比较模型鼠血、尿中IL-17升高(P<0.01);IL-17仅在糖尿病大鼠肾小管-间质中表达,且肾组织IL-17表达与尿中IL-17、UAER及CRP水平呈正相关关系。结论:IL-17在糖尿病大鼠肾组织表达明显增加,在糖尿病肾小管-间质病变中可能起重要作用。To investigate the expression of interleukin-17 in renal tissue of diabetic rats. Methods： Ninety male Sprague-Dawley rats were randomly divided into three groups： control group, type 1 diabetic rat group （T1DM group）, and type 2 diabetic rat group （T2DM group）. T1DM group was induced by intraperitoneal injection of streptozoticin （STZ, 50 mg/kg）. T2DM group was fed with a diet enriched with sucrose, triglyceride and cholesterol, and STZ （25 mg/ kg） was injected intraperitoneally. At the end of the 2nd and 4th weeks, serum and urine IL-17 were measured with enzyme-linked immunosorhent assay （ELISA）. Meantime, the urinary albu- min excretive rate （UAER） and C-reactive protein （CRP） were measured. The expression of IL- l7 in the kidney was assessed by immunohistochemisty staining and Western blotting. Results： Serum and urine IL-17 showed obvious increase in diabetic rats. The expression of IL-17 was only observed in renal tubulointerstitium of diabetic rats, and the expression of IL-17 in renal tubu- lointerstitium of diabetic rats was positively correlated with urine IL-17, UAER, and CRP. Conclusion： The aberrant increase of IL-17 expression may play an important role in tubulointer- stitium inflammation of diabetic rats.

关 键 词：白细胞介素-17 糖尿病 肾小管-间质 

分 类 号：R587.2[医药卫生—内分泌]