厄贝沙坦联合雷公藤甲素对2型糖尿病大鼠肾脏ANGPTL2、VEGF表达影响  

Influence of Irbesrtan Combined with Triptolide on ANGPTL2 and VEGF in the Kidney of 2 Type Diabetes Rat Models

作　　者：李柯桢 栾健[2] 隋爱华[3] 马瑞霞[4] 周丽敏[4] 王艳[4] 

机构地区：[1]青岛大学医学院,硕士研究生青岛266000 [2]山东省青岛市市立医院内分泌科,青岛266000 [3]青岛大学医学院附属医院中心实验室,青岛266000 [4]青岛大学医学院附属医院肾内科,青岛266000

出　　处：《中国中西医结合肾病杂志》2013年第11期959-962,I0003,I0004,共6页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：青岛市科技局项目(No.2012-1-3-1-(19)-nsh)

摘　　要：目的:观察厄贝沙坦联合雷公藤甲素(TP)对ANGPTL2、VEGF在2型糖尿病(T2DM)大鼠肾脏表达的影响并探讨其机制。方法:50只SD雄性大鼠随机分为正常对照组(NC,10只)和实验组(40只),实验组给予高糖高脂喂养联合小剂量链脲佐菌素(STZ 30 mg/kg)建立T2DM大鼠模型,再随机分为4组:糖尿病对照组(DM)、厄贝沙坦组(DI)、雷公藤甲素组(DT)及厄贝沙坦联合雷公藤甲素组(DIT)。药物干预8周后,测体重、血压、肾重、血糖、血肌酐(Scr)、尿素氮(BUN),24 h尿蛋白(UAL)的排泄量;镜下观察肾组织病理改变;免疫组化显色技术及实时定量PCR检测血管生成素样蛋白2(ANGPTL2)、血管内皮生长因子(VEGF)表达的变化。结果:DM组与NC组相比血糖、肌酐、BUN及UAL明显升高(P<0.01),实时定量PCR示ANGPTL2、VEGF mRNA明显上调(P<0.01);与DM组相比较,DT、DI及DIT组血糖、肌酐、BUN及UAL明显降低(P<0.01),DT、DI组中ANGPTL2、VEGF mRNA表达下调,DIT组更为明显(P<0.05)。结论:ANGPTL2、VEGF在T2DM大鼠肾脏中的表达上调,厄贝沙坦及雷公藤甲素可降低ANGPTL2及VEGF在T2DM大鼠肾脏中的表达,并有协同作用。Objective：To observe the effect of irbesartan combined with triptolide on ANGPTL2 and VEGF in the kidney of 2 type diabetes（T2DM） rat models, and discuss its mechanism. Methods： 50 SD male rats were divided into normal control group （NC,n = 10） and experimental group （n =40） randomly . The experimental group was fed with high sugar -fat diet and given a low dose streptozocin（STZ 30 mg/kg） established type 2 diabetic model rats, and then randomly divided into 4 groups：diabetes control group （DM） ,irbesrtan group（DI） ,triptolide group（DT） and irbesartan combined with triptolide group （DIT）. Weight, blood pres- sure, blood glucose, serum creatinine（ Scr）, blood urea nitrogen（ BUN）, 24 hour urinary albumin（ （UAL） and renal histomorphology were observed after Drug intervention for 8 weeks, the expression of angiotensin - like protein 2 （ ANGPTL2）, vascular endothelial growth factor（VEGF） in renal tissue were detected by immunohistochemisty and real -time PCR. Results：blood glucose, serum creati- nine, BUN and UAL in DM group were obviously higher than those of the NC group （P 〈0.01 ） ,and the mRNA of ANGPTL2 and VEGF distantly upregulated compared with NC group （P 〈 0.01 ） ; Meanwhile blood glucose, serum creatinine and BUN and UAL in DI,DT and DIT group were significantly lower（P 〈0.01 ）compared to DM group,and the mRNA of ANGPTL2 and VEGF downregu- lated. Neverthless, in irbesrtan combined with triptolide group the changes of those indexes above were more significant than those in DI group or in DT group （ P 〈 0.05 ）. Conclusion： These results indicate that the expression of ANGPTL2, VEGF in kidney tissue of T2DM rats were upregulated ;irbesartan and triptolide could decrease ANGPTL2 ,VEGF,and they have synergistic effect.

关 键 词：血管生成素样蛋白2 血管内皮生长因子 雷公藤甲素 厄贝沙坦 糖尿病肾病 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]