低温等离子射频消融技术对猪感染创面的在体杀菌作用  

Bactericidal effects of low-temperature plasma radiofrequency ablation technique on bacteria infected wounds in minipigs in vivo

作　　者：杨润功[1] 左坦坦[1] 朱加亮[1] 张宇鹏[1] 任刚[1] 侯树勋[1] 

机构地区：[1]解放军总医院第一附属医院骨科,北京100048

出　　处：《中国骨与关节杂志》2013年第12期707-711,共5页Chinese Journal of Bone and Joint

摘　　要：目的观察低温等离子对不同细菌的在体杀菌效果。方法选取5头小型猪,每只猪背部使用特制打孔器制备36个直径为9 mm(面积约为0.64 cm^2)、深度约为0.3 cm的圆形创面,分别为每个创面接种0.05ml浓度为0.5×10~8 CFU/ml的金黄色葡萄球菌稀释液、表皮葡萄球菌稀释液或铜绿假单胞菌稀释液,制成全层皮肤缺损感染创面的动物模型。每种细菌感染的60个创面采用数字随机法分为10组,3档5 s组、3档10 s组、3档15 s组、6档5 s组、6档10 s组、6档15 s组、9档5 s组、9档10 s组、9档15 s组和未处理组。每组6个创面。然后对创面进行细菌定量检查并进行统计学分析。结果 180个创面接种金黄色葡萄球菌、表皮葡萄球菌和铜绿假单胞菌稀释液后48 h,均制成感染性创面。经过等离子射频处理后的各种细菌感染创面细菌量均较未处理的创面有明显降低,金葡菌3档0.0034;金葡菌6档0.0128;金葡菌9档0.0058。表皮葡萄球菌3档0.0213;表皮葡萄球菌6档0.0005;表皮葡萄球菌9档0.0012。铜绿假单胞菌3档0.0318;铜绿假单胞菌6档0.0085;铜绿假单胞菌9档0.0125,差异有统计学意义(P<0.05);各档位的不同作用时间相比较,差异有统计学意义(P<0.05),作用时间越长,杀菌率越高;各作用时间的3档和6档、9档比较,差异有统计学意义(P<0.05),且档位越高,杀菌作用越明显。6档和9档之间比较差异不明显。结论低温等离子射频消融术对多种不同临床常见细菌均具有明显的在体杀菌作用。Objective To observe the bactericidal effects of low-temperature plasma technique on different bacteria infected wounds in vivo. Methods Infected wounds of full-thickness skin defects were prepared in 5 minipigs, and 36 round wounds were made on the back of each minipig using specially made puncher. The diameter of each wound was 9 mm （ the area was approximately 0.64 cm2 ）, and the depth was about 0.3 cm. Each wound was inoculated with 0.05 ml staphylococcus aureus （ SA ）, staphylococcus epidermidis （ SE ） and pseudomonas aeruginosa （ PA ） dilution, whose concentration was 0.ix i0s CFU/mI. 60 wounds infected by each of these bacteria were divided into 10 groups randomly. There were the strength 3 and 5 s group, the strength 3 and 10 s group, the strength 3 and 15 s group, the strength 6 and 5 s group, the strength 6 and 10 s group, the strength 6 and 15 s group, the strength 9 and 5 s group, the strength 9 and 10 s group, the strength 9 and 15 s group and the control group, with 6 wounds in each group. The quantitative bacterial examination of wounds in each group was carried out, and statistical analysis was performed. Results 180 infected wounds were made at 48 hours after they were inoculated with SA, SE and PA dilution, The bacterial counts in the groups treated by plasma radiofrequency ablation technique were significantly decreased when compared with that in the control group, which were 0.0034, 0.0128 and 0.0058 in the strength 3, 6 and 9 groups inoculated with SA, 0.0213, 0.0005 and 0.0012 in the strength 3, 6 and 9 groups inoculated with SE and 0.0318, 0.0085 and 0.0125 in the strength 3, 6 and 9 groups inoculated with PA, with statistically significant differences （ P〈0.05 ）. Statistical differences also existed in each strength group for different durations of operation. The longer the duration of operation was, the higher the sterilization rate became. After the same duration of operation, there were statistically significant differences among the strength 3, 6 and 9 groups. The

关 键 词：细菌感染 等离子气体 抗感染药 局部 消融技术 

分 类 号：R641[医药卫生—外科学]