MCP-1对糖尿病小鼠创面愈合的促进作用  

Monocyte chemoattractant protein 1 helping wound healing in diabetic mice

作　　者：彭银波[1,2] 卢化祥 姚敏[1,2] 方勇 顾钏[1,2,3] 

机构地区：[1]上海交通大学医学院附属第三人民医院烧伤整形科 [2]上海交通大学医学院创伤研究所,上海201900 [3]上海交通大学医学院附属第九人民医院整复外科,上海200011

出　　处：《上海交通大学学报（医学版）》2013年第12期1583-1586,1590,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市卫生局青年基金项目(2008Y063);上海交通大学医学院院级基金(11XJ21051);国家自然科学基金青年科学基金项目(81101431)~~

摘　　要：目的探讨早期使用外源性单核细胞趋化蛋白1(MCP-1)对糖尿病小鼠创面愈合的影响及可能机制。方法 60只BKS糖尿病基因小鼠随机分为MCP-1组和对照组(n=30)。在小鼠背部正中制作1 cm×1 cm大小创面,沿深筋膜去除全层皮肤。MCP-1组和对照组小鼠创面局部分别予以MCP-1(50 ng/d)和等体积PBS,持续3 d,于伤后第3、5、7、10、14天,计算创面愈合率,检测创面胶原蛋白标志性成分羟脯氨酸(OHP)含量、新生血管数和血管内皮生长因子(VEGF)的表达。结果从伤后第5天起,MCP-1组的创面愈合率显著高于对照组(P<0.05);MCP-1组各观察时相点创面组织中OHP的含量均显著高于对照组(P<0.05)。伤后第5、7天,MCP-1组和对照组创面每高倍视野新生血管数分别为5.25±0.83和3.2±0.52及7.46±0.69和4.8±0.81,组间比较差异均有统计学意义(P<0.01);伤后第5、7、10天,MCP-1组创面VEGF的表达均显著高于对照组(P<0.05)。结论早期使用外源性MCP-1可加快糖尿病小鼠创面愈合,其作用可能与促进血管新生及胶原合成有关。Objective To diabetic mice. Methods assess effects of exogenous Sixty BSK type 2 diabetic monocyte ehemoattraetant protein mice were randomly divided into 1 （MCP-1） in wound healing of MCP-1 treated group and control group （30 in each）. An excision wound down to the deep fascia, measuring 1 cm x 1 cm, was made on the back of mice. The MCP-1 group mice were injected with MCP-I （50 ng/d） for 3 d, and the control group mice received equal volume of PBS. The rate of wound healing, deposition of hydroxyproline （OHP）, new blood vessels, and the level of vascular endothelial growth factor （VEGF） were assessed on day 3, 5, 7, 10, and 14 after injury. Results Since day 5, compared to control group, the rate of wound healing and deposition of OHP were higher in MCP-ltreated group （P 〈 0.05）. On day 5 and 7, the new blood vessels per high power field were 5.25 ±0.83 vs 3.2 ±0.52 and 7.46 ±0.69 vs 4.8 ±0.81 respectively （P 〈0.01） in MCP-ltreated group and control group. Additionally, the levels of VEGF in MCP-1 group were significantly higher than those in control group on day 5, 7, and 10. Conclusion Exogenous MCP-1 may promote diabetic wound healing by enhancing the production of collagen and angiogenesis.

关 键 词：创面愈合 单核细胞趋化蛋白1 血管新生 胶原合成 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]