acs-20;acs-22双突变线虫对低渗溶液及小分子药物的敏感性研究  

Sensitivity of acs-20; acs-22 double mutant elegans for hypotonic solution and small molecule compounds

作　　者：王会子 韩冰[2] 高耀辉 王立顺[2] 夏薇[1] 

机构地区：[1]北华大学医学检验学院,吉林132013 [2]上海交通大学医学院附属瑞金医院分子医学中心,上海200025 [3]上海市闵行区中心医院科教科,上海201100

出　　处：《上海交通大学学报（医学版）》2013年第12期1591-1595,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的分析acs-20;acs-22双突变线虫是否适合经皮肤渗透吸收的小分子化合物的体内效应研究,为建立药物筛选的生物新模型提供线索。方法野生型秀丽隐杆线虫N2作为对照,PCR验证acs-20;acs-22双突变线虫2个突变基因(acs-20和acs-22)的缺失。将2种线虫浸泡于低渗溶液(双蒸水)中,记录2种线虫浸后20、40、60 min时间点的生存率,对比2种线虫对液体渗透压的敏感性。分别用10、20μg/mL的阿霉素(DOX)处理线虫,记录2种线虫在药物处理后12、24、48 h时间点的生存率,对比2种线虫对毒性药物的敏感性。结果 PCR结果显示:与野生型秀丽隐杆线虫N2相比,在acs-20;acs-22双突变线虫DNA序列中确实存在acs-20和acs-22基因缺失,acs-20;acs-22双突变线虫对低渗液体环境和DOX更为敏感。结论与野生型秀丽隐杆线虫相比,acs-20;acs-22双突变线虫更适合观察经皮肤渗透吸收的小分子化合物的体内效应,可以发展成为一种新的药物筛选生物模型。Objective To investigate acs-20; acs-22 double mutant elegans sensitivity to small molecular compound absorbed through skin pelletration, and to provide new model for drug screening. Methods Wild type Caenorhabditis elegans N2 （C. elegans N2） as control, acs-20; acs-22 double mutant elegans was verified by PCR. Both wild type and ＋ acs-20; acs-22 double mutant elegans were soaked in hypotonic solution （double distilled water）. The survival rates were recorded at the time of 20, 40, and 60 min to compare the sensitivity of both kinds of elegans to the changes of osmotic pressure. Both elegans were treated with DOX （10 mg/mL and 20 mg/mL） and the survival rates were recorded at the time of 12, 24 and 48 h after treatment. Results PCR results showed that the two relevant genes were successfully mutated in acs-20; acs-22 double mutant elegans compared with the wild type C. elegans N2. And acs-20; acs-22 double mutant elegans was more sensitive to hypotonic solution and DOX treatment. Conclusion Compared to wild type C. elegans N2, acs-20; acs-22 double mutant elegans is more suitable for observing in vivo effect of small molecular compound absorbed through skin penetration, and more appropriate to be used as a new model animal for drug screening.

关 键 词：acs-20 acs-22双突变线虫 模型 药物筛选 

分 类 号：R965.1[医药卫生—药理学]