锌对砷致雄性大鼠睾丸毒性损伤的拮抗作用  

Antagonism of zinc on testicular toxicity induced by arsenic exposure in male rats

作　　者：南楠[1] 詹平[1] 

机构地区：[1]重庆医科大学公共卫生与管理学院劳动卫生与环境卫生教研室,重庆400016

出　　处：《上海交通大学学报（医学版）》2013年第12期1637-1642,共6页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的探讨不同剂量的锌对砷的睾丸毒性的拮抗作用。方法将50只Sprague-Dawley(SD)雄性大鼠随机分为对照组、单纯染砷组(4 mg/kg NaAsO2)、砷加低锌组(4 mg/kg NaAsO2+8 mg/kg ZnSO4)、砷加中锌组(4 mg/kg NaAsO2+20 mg/kg ZnSO4)、砷加高锌组(4 mg/kg NaAsO2+50 mg/kg ZnSO4)。连续灌胃60 d后,对睾丸进行苏木精-伊红(HE)染色,观察睾丸组织形态学变化;免疫组织化学法测Fas蛋白表达;放射免疫法测血清睾酮含量;原子吸收法测睾丸锌含量;ELISA测睾丸丙二醛(MDA)含量和乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活性。结果单纯染砷组大鼠与对照组相比,生精细胞减少,出现空泡,Fas蛋白表达增多,睾丸锌含量下降,睾酮水平下降,脂质过氧化损伤增强(P<0.05)。砷加锌的各组大鼠与单纯染砷组相比,Fas蛋白表达降低,睾酮含量升高,脂质过氧化情况明显好转(P<0.05),其中砷加中锌组保护效果最好;砷加低锌组与单纯染砷组相比,MDA和LDH无显著差异。结论砷染毒可造成睾丸组织损伤,一定程度上引起了生殖系统功能障碍,适当剂量的锌可以拮抗这种毒性作用。Objective To explore the antagonism effects of different doses of zinc on testicnlar toxicity induced by arsenic. Methods Fifty male Sprague-Dawley rats were randomly divided into five groups： control group, arsenic group （4 mg/kg NaAsO2）, arsenic plus low-dose zinc group （4 mg/kg NaAsO2 ＋ 8 mg/kg ZnSO4）, arsenic plus mid-dose zinc group （4 mg/kg NaAsO2 ＋ 20 mg/kg ZnSQ）, and arsenic plus high-dose zinc group （4 mg/kg NaAsO2 ＋ 50 mg/kg ZnSO4）. Intragastric administration was applied for 60 consecutive days. The testes were observed with hematoxylin-eosin （HE） staining. The expression of Fas protein was examined by immunohistochemistry staining. The concentration of testosterone in serum was examined by radioimmunoassay. The zinc content in testis was determined by atomic absorbing spectrometry. The levels of malondialdehyde （MDA）, lactic dehydrogenase （LDH）, and superoxide dismutase （SOD） were measured by enzyme-linked immunosorbent assay （ELISA）. Results Compared to control group, arsenic administration caused remarkable decreases of spermatogenic ceils and vacuolization of seminiferous tubular cells, and higher expression of Fas, significant reduction of zinc content, lower testosterone concentration, and significantly aggravated lipid peroxidation were observed in arsenic group （P 〈 0.05）. Compared to arsenic group, zinc treatment significantly reduced the expression of Fas, increased serum testosterone level, and alleviated lipid peroxidation （P 〈 0.05）. The protective effect of mid-dose zinc was the best. There were no differences in the levels of MDA and LDH between arsenic group and arsenic plus low-dose zinc group. Conclusion Arsenic can lead to testis damage which may cause reproductive functional impairment. Zinc treatment can antagonize the toxicity of arsenic when the dosage of zinc is suitable.

关 键 词：砷 锌 睾酮 凋亡 脂质过氧化 

分 类 号：R363[医药卫生—病理学]