哺乳动物精子获能前后的理化变化及获能机制研究进展  

Advance of Capacitation Mechanism and the Physiochemical Changes of Sperm before and after Capacitation of Mammalian

作　　者：贺亚南 朱化彬[1] 陈晓丽[1] 郝海生[1] 秦彤[1] 赵学明[1] 路永强 王栋[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室,北京100193 [2]北京畜牧兽医总站,北京100107

出　　处：《畜牧兽医学报》2013年第12期1867-1873,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"十二五"科技支撑计划(2011BAD19B02);奶牛产业技术体系北京创新团队项目

摘　　要：综述了精子获能前后的运动轨迹和质膜微观结构的改变,及与之相伴的代谢类型、酶系活性和相关离子流动等一系列理化变化,并从胆固醇外流、活性氧产生、蛋白质磷酸化、膜电位极化等方面阐述了获能机理,同时指出,蛋白酪氨酸磷酸化是精子获能的关键。提出应用转录组学、蛋白组学结合生物信息学等技术,研究获能过程胆固醇转运体的作用方式、胞外Ca2+对获能的影响、与质膜胆固醇流失相伴的膜外Ca2+、HCO-3内流顺序及其关系、与信号传导相关蛋白的酪氨酸磷酸化等重要获能事件,将为深入研究精子获能机制提供一定的理论参考。Before and after sperm capacitation, the motion trail and membrane microstructure were changed, and associated physiochemical change events of the metabolic types, enzyme activ- ity and fluidity of related ions. In addition, we also reviewed the mechanism of capaeitation from the cholesterol efflux, production of reactive oxygen species （ROX）, protein tyrosine phospho- rylation （PTP） and variation membrane potential. Meanwhile, the importance of PTP during sperm capacitation was pointed out in current review. We offered that the combined use of bioin- formatics tools and proteomie/transeriptomics would be applicated on the study about cholesterol efflux from plasma membranes to acceptors mediated by the transporters, the effect of extracellu- lar Ca2＋ on capacitation, the inflow turn of extracellular Ca2＋ and HC03 accompanied with the cholesterol efflux and their relationship, as well as the PTP related to the signaling pathway, which could provide a theoretical guidance for further study on capacitation mechanism.

关 键 词：精子获能 蛋白酪氨酸磷酸化 获能机制 

分 类 号：S814.8[农业科学—畜牧学]