高、低脂鸡脂肪组织ATGL基因的表达及其激素调控的差异分析  

Adipose Tissue Expression and Hormonal Regulation of ATGLGene in the Fat and Lean Broiler Lines

作　　者：史铭欣[1,2,3] 荣恩光[1,2,3] 周纬男[1,2,3] 史洪岩[1,2,3] 李辉[1,2,3] 王宁[1,2,3] 

机构地区：[1]农业部鸡遗传育种重点实验室,哈尔滨150030 [2]黑龙江省普通高等学校动物遗传育种与繁殖重点实验室,哈尔滨150030 [3]东北农业大学动物科学技术学院,哈尔滨150030

出　　处：《畜牧兽医学报》2013年第12期1874-1881,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展计划(973课题)(2009CB941604);黑龙江省教育厅家禽遗传育种科技创新团队(2010td02)

摘　　要：为研究脂肪甘油三酯脂肪酶（ATGL）在鸡脂肪沉积中的作用，并分析肾上腺素和胰高血糖素对高、低脂鸡脂肪组织和脂肪细胞ATGL基因表达的影响，本研究利用半定量RT-PCR分析鸡ATGL基因的组织表达谱；利用Real-timeRT-PCR分析ATGL基因及其活化因子Comparative gene identification-58（CGI-58）基因在1～7周龄东北农业大学肉鸡高、低腹脂双向选择品系（NEAUHLF）第16世代鸡群脂肪组织中的表达差异，以及肾上腺素和胰高血糖素对高、低脂系肉鸡脂肪组织和脂肪细胞ATGL基因表达的影响。结果显示，ATGL基因在鸡腹部脂肪、皮下脂肪、嗉囊周围脂肪、肌胃周围脂肪、肾脏及肝脏中表达量较高；低脂系肉鸡腹部脂肪组织ATGL和CGI-58基因的表达总体均高于高脂系鸡，且均在第2周龄达到显著性差异（P％0．05）；肾上腺素和胰高血糖素都能够上调高脂系鸡脂肪组织和脂肪细胞ATGL基因的表达，并促进机体的脂肪分解，暗示高脂系鸡对2种激素的刺激相对敏感。本研究结果为揭示高、低脂肉鸡脂肪性状差异的原因以及ATGL在鸡脂肪沉积中的作用奠定了基础。Abstract. This study aimed to investigate the function of adipose triglyceride lipase（ATGL）in chicken fat deposition, and to analyze the effects of adrenaline and glucagon on the gene expres- sion of ATGL in adipose tissue and adipocytes of the fat and lean broilers. The tissue expression pattern of ATGL gene was analyzed by semi-quantitative RT-PCR （SqRT-PCR）. The expression of ATGL gene and its activator Comparative gene identification 58 （CGI-58） gene in abdominal adipose tissue and adipocytes in the fat and lean broiler lines of the 16th generation of NEAUHLF at 1-7 week-old was analyzed by Real-time RT-PCR. The results showed that chicken ATGL gene was highly expressed in abdominal fat, subcutaneous fat, crop fat, kidney, liver and gizzard fat. On the whole, the mRNA expression levels of ATGL and CGI-58 genes were higher in the leanbroiler lines than that in the fat broiler lines. In particular, the expression of ATGL and CGI-58 genes in the lean broiler lines was significantly higher than that in the fat broiler lines at 2 week- old （P〈0.05）. Adrenaline and glucagon up-regulated the expression of ATGL gene in the ab- dominal adipose tissue and adipocytes of the fat broiler lines and promoted lipolysis, which indica- ted that the fat broiler lines were more sensitive to the both hormones than the lean broiler lines. This study not only laid the foundation for revealing the difference of the fat phenotype of fat and lean broiler lines, but also offered a basis for clarifying the function of ATGL in fat deposition in broiler.

关 键 词：肉鸡 ATGL基因 基因表达 肾上腺素 胰高血糖素 

分 类 号：S831.2[农业科学—畜牧学]