沙葱多糖对绵羊外周血淋巴细胞IFN-γ与STAT1 mRNA表达的影响  

Effect of Allium mongolicum Regel Polysaccharides on IFN-γand STAT1 mRNA Expression in Peripheral Blood Lymphocyte of Sheep

作　　者：赵飞艳[1] 敖长金[1] 包美艳[1] 萨茹丽[1] 

机构地区：[1]内蒙古农业大学动物科学学院,呼和浩特010018

出　　处：《畜牧兽医学报》2013年第12期1932-1938,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30760173)

摘　　要：本研究在体外条件下研究了沙葱多糖对绵羊外周血淋巴细胞IFN-γ与STAT1mRNA表达的影响,旨在从分子水平上探讨沙葱多糖的抗肿瘤作用机制。试验用不同浓度的沙葱多糖(0、50、100、150、200、250μg·mL-1)体外作用于绵羊外周血淋巴细胞,作用持续12、24、48、72h后分别提取细胞的总RNA,以β-actin作为内参基因,用实时荧光定量RT-PCR方法测定IFN-γ与STAT1mRNA相对表达量的变化,并对I FN-γ与STAT1的PCR产物进行测序分析。结果显示,沙葱多糖能够显著提高IFN-γ与STAT1mRNA相对表达量(P<0.05);随着沙葱多糖浓度的升高,IFN-γ与STAT1mRNA相对表达量基本呈升高的趋势,且测序分析结果证明扩增产物为羊的IFN-γ与STAT1基因序列。结果表明,沙葱多糖可显著促进绵羊外周血淋巴细胞IFN-γ与STAT1mRNA的表达,进而发挥其抗肿瘤作用。The experiment was conducted to γstudy the effects of Allium rnongolicum Regel poly- saccharides on the mRNA expression of IFN-, and STAT1 in peripheral blood lymphocyte in vitro ,and to discuss the possible molecular mechanism of its anti-tumor function in sheep. Differ ent concentrations （0, 50, 100, 150, 200, 250 μg· mL^（-1） ） of Allium mongolicum Regel polysac- eharides were added in sheep peripheral blood lymphocytes cultures, and total RNA in cells was extracted with fl-actin as an internal reference real-time PCR was adopt to test. The changes of relative mRNA expressions of IFN-γ, and STAT1 in different culrured times （12, 24, 48, 72 h） were tested by real-time PCR and the PCR products of IFN-γ, and STAT1 were sequenced and analyzed. The relative mRNA expressions of IFN-γ and STAT1 in Allium mongolicum Regel polysaccharides treatment were increased significantly （P〈0.05） compared with control group （0 μg ~ mL· （-1） ）, and showed an upward trend with the increase of Allium mongolicum Regel poly- saccharides concentration. The sequencing analysis proved that the PCR products were IFN-γ and STAT1 gene sequence in sheep. The results suggested that Allium mongolicum Rege! poly saccharides could significantly promote the mRNA expression of IFN-γ, and STAT1 in sheep pe- ripheral blood lymphocytes of sheep, may thereby play an important role in anti-tumor effect.

关 键 词：沙葱多糖 淋巴细胞 RT—PCR 干扰素Γ 信号转导与转录激活因子1 

分 类 号：S826[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]