金黄色葡萄球菌外毒素及荚膜特异性卵黄抗体联合治疗奶牛乳房炎效果研究  

Efficacy of a Combination of Egg Yolk Immunoglobulins against Staphylococcus aureus Exotoxins and Capsules in the Treatment of Bovine Mastitis

作　　者：王林会[1] 李晓宇[1] 金礼吉[1,2] 李淑英[2] 徐永平[1,2] 

机构地区：[1]大连理工大学生命科学与技术学院,大连116024 [2]动物性食品安全保障技术教育部工程研究中心,大连116020

出　　处：《畜牧兽医学报》2013年第12期2016-2021,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：中央高校基本科研业务费(DUT13JB04);国家自然科学基金(30371053;31001053)

摘　　要：本研究目的是评价金黄色葡萄球菌外毒素特异性卵黄抗体(IgY-toxins)及5、8、336型金黄色葡萄球菌荚膜特异性卵黄抗体(IgY-T5、IgY-T8及IgY-T336)联合使用对奶牛乳房炎的治疗效果。乳酸脱氢酶试验表明,IgYtoxins能够有效中和外毒素对乳腺上皮细胞MAC-T的破坏作用。荧光显微镜观察发现,5mg·mL-1的IgYT336可有效阻断金黄色葡萄球菌RN6390对MAC-T的侵袭。与单独使用IgY-T336相比,5mg·mL-1的IgYT336和0.5mg·mL-1的IgY-toxins联合使用能够更加有效地保护MAC-T细胞,同时显著改善细胞的生长形态。临床型乳房炎试验表明,与PBS对照相比,对金黄色葡萄球菌感染乳池每天注射各20mg的IgY-T5、IgY-T8、IgYT336及IgY-toxins可显著降低(P<0.001)病牛乳汁的体细胞数(SCC),20mg的IgY-T5、IgY-T8、IgY-T336或者20mg的IgY-toxins虽然也可降低体细胞数,但是降低幅度显著低于联合治疗组(P<0.01)。所有治疗组奶牛乳汁的凝块水平都显著低于对照组(P<0.05)。本研究表明,荚膜及外毒素特异性卵黄抗体联合使用比其单独使用对乳房炎的治疗效果更好,在应用卵黄抗体治疗乳房炎时应该将荚膜和外毒素都列为抗体的靶点。The objective of this study was to evaluate the efficacy of a combination of egg yolk im- munoglobulins （IgYs） specific for exotoxins （IgY-toxins） and serotypes 5, 8 and 336 capsules of Staphylococcus aureus （IgY-TS, IgY-T8, IgY-T336, respectively） in the treatment of bovine mastitis. A lactate dehydrogenase assay indicated that IgY-toxins prevented mammary epithelial cells （MAC-T） from lysing. An internalization assay demonstrated that 5 mg ~ mL-1 IgY-T336 markedly blocked the invasion of a GFP-expressing S. aureus RN6390 to MAC-T cells, and a combination of 5 mg · mL-1 of IgY-T336 and 0. 5 mg · mL-1 of IgY-toxins provided the cells with greater protection and improved growth morphology compared with the IgY-T336 treatment alone. A field trial for clinical mastitis indicated that two doses of a combination of 20 mg each of IgY TS, IgY-T8 and IgY-T336 plus 20 mg of IgY-toxins daily for six days significantly reducedsomatic cell counts （SCC） compared with PBS control （P ~ 0. 001）. Treatment with IgY-T5, IgY-T8 and IgY-T336 or IgY-toxins alone produced a slight reduction in SCC compared with PBS control. However, the reduction was much lower than that in IgY combination treatment （P 0.01）. Both surface capsules and secreted exotoxins should be targeted in the treatment of S. au- reus-caused bovine mastitis with IgY.

关 键 词：金黄色葡萄球菌 外毒素 卵黄抗体 乳房炎 体细胞计数 

分 类 号：S857.26[农业科学—临床兽医学]