四君子汤对脾虚大鼠肠道菌群多样性的影响  

Influence of Sijunzi Decoction on Intestinal Flora Diversity in Spleen-deficient Rats

作　　者：孟良艳[1] 陈秀琴[1] 石达友[1] 黄海定[1,2] 郭世宁[1] 

机构地区：[1]华南农业大学兽医学院,广州510642 [2]广东省中医院,广州510405

出　　处：《畜牧兽医学报》2013年第12期2029-2035,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：十二五农村领域国家科技计划课程(2011BAD34B01-14);广东省教育部产学研合作项目(2011B09040026)

摘　　要：探讨四君子汤对脾虚大鼠肠道菌群多样性的影响。将48只大鼠随机分为空白组、模型组、中药四君子汤组、柳氮磺吡啶肠溶片(SASP)组,每组12只。将除空白组外的其余各组大鼠用利血平法造脾虚模型。然后给四君子汤、SASP分别灌胃治疗后,通过16SrDNA测序法分析肠道菌群多样性变化。结果表明:与模型组比较,四君子汤组中的肠道菌群多样性明显升高,但与西药组中的肠道菌群多样性有差异。四君子汤能通过改善肠道菌群的多样性调理脾虚证。The aim of this study was to investigate and analyze the recuperative function of Chi- nese Medicine, Sijunzi decoction, on intestinal flora in spleen-deficient animals. Forty-eight rats were randomly divided into the Control group （n=12）, the Spleen-deficient group （n=12）, the Sijunzi decoction group （n=12） and the Sulfasalazine group （n= 12）. Spleen-deficiency rat model was caused by reserpine in all groups other than the Control group. A diarrhea model of rats was conducted followed by treatment of sijunzi decoction or SASP or non-treatment. Intestinal flora was determined at pre-modeling, pre- and post- treatment by a 16S rDNA gene sequencing. The results showed that the Sijunzi decoction improved the diversity of intestinal flora in diarrhea rats though the diversity of intestinal flora differs from that treated with SASP. The Sijunzi decoction is a potential medicine for treatment of intestinal flora disorder induced diarrhea.

关 键 词：四君子汤 脾虚 肠道菌群 16S RDNA 

分 类 号：R285.5[医药卫生—中药学]