间日疟原虫环子孢子蛋白PCR-RFLP多态性分析  

Polymorphism Analysis on the Genotypes of Circumsporozoite Protein of Plasmodium vivax with PRC-RFLP

作　　者：刘颖[1] 周瑞敏[1] 陈伟奇[1] 钱丹[1] 赵玉玲[1] 赵旭东[1] 许汴利[1] 苏云普[1] 张红卫[1] 

机构地区：[1]河南省疾病预防控制中心,郑州450016

出　　处：《中国寄生虫学与寄生虫病杂志》2013年第6期483-485,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：采集2011年河南省间日疟原虫阳性患者血样,用限制性片段长度多态性聚合酶链反应(PCR-RFLP)法分析环子孢子蛋白(circumsporozoite protein,CSP)基因型,并结合流行病学资料进行初步种群分布分析。共采集间日疟患者血样37份,根据流行病学调查确定河南当地感染病例血样26份,输入性病例血样11份。37份血样均扩增出1条约750 bp的条带。PCR-RFLP结果显示,CSP基因PV-Ⅱ型1份,占2.7%,PV-Ⅰ型36份,占97.3%。26份当地病例血样CSP基因均为PV-Ⅰ型,其中PV-Ⅰ-a型占42.3%(11/26),PV-Ⅰ-b型占57.7%(15/26);11份输入病例血样中,PV-Ⅰ-a型占90.9%(10/11),PV-Ⅱ型占9.1%(1/11),未见PV-Ⅰ-b型。提示河南省当地流行的间日疟病例中,CSP基因PV-Ⅰ-b型略多于PV-Ⅰ-a型;输入性间日疟病例中PV-Ⅰ-a型占优势。Thirty-seven blood samples from Plasmodium vivax-infected patients were collected in Henan Province in 2011. The gene for circumsporozoite protein （PvCSP） was amplified by nested PCR. According to the epidemiological data, among the 37 Plasmodium vivax malaria cases, 26 were indigenous cases and 11 were imported cases. A fragment of 750 bp was obtained in the 37 blood samples. The 750 bp PCR product was digested with restr/ction endonuclease Alu- I and Mva-I, and genetic polymorphism was investigated with PCR-RFLP. PCR-RFLP analysis of PvCSP revealed that the parasites were PV-I type （97.3%, 36/37） and PV-II type （2.7%, 1/37）. 26 samples from indigenous cases were exclusively PV-I type with PV-I-b type （57.7%, 15/26） and PV-I-a type （42.3%, 11/26）. Among 11 samples from imported cases, PV-I-a type accounted for 90.9% （10/11）, and 1 PV-II type accounted for 9.1% （1/11）, no PV-I-b type were detected.

关 键 词：河南 间日疟原虫 环子孢子蛋白 基因型 

分 类 号：R382.311[医药卫生—医学寄生虫学]