手足口病患儿Th17、CD4^+CD25^+调节性T细胞的变化及意义  

Changes in Th17 and CD4^+CD25^+ Treg cells and their significance among children with hand,foot and mouth disease

作　　者：姜涛[1] 李双杰[1] 欧阳文献[1] 谭艳芳[1] 刘芙蓉[1] 肖耿吉[1] 唐莲[1] 张慧[1] 

机构地区：[1]湖南省儿童医院肝病中心,湖南长沙410007

出　　处：《中国当代儿科杂志》2013年第12期1113-1115,共3页Chinese Journal of Contemporary Pediatrics

基　　金：湖南省中医管理局项目(201202)

摘　　要：目的研究手足口病患儿外周血Th17、CD4+CD25+调节性T细胞(CD4+CD25+Treg)的变化。方法以89例手足口病患儿为研究对象,其中普通型55例,重型34例,另选取30例健康儿童为对照组;采用流式细胞术检测各组外周血CD4+CD25+Treg细胞、Th17细胞在CD4+T淋巴细胞中所占的比例;采用ELISA法检测IL-10、TGF-β和IL-17在各组的表达水平。结果与对照组相比,重型和普通型手足口病患儿的Th17细胞、IL-17水平均明显升高(均P<0.05),而CD4+CD25+Treg细胞、IL-10、TGF-β水平则显著下降(均P<0.05),且手足口病患儿外周血Th17、IL-17水平随病情严重而增高,而CD4+CD25+Treg细胞、IL-10、TGF-β水平随病情严重而降低。结论手足口病患儿外周血Th17细胞应答增强,而CD4+CD25+Treg细胞应答降低,Th17细胞与CD4+CD25+Treg细胞比例失衡可能在手足口病发病机制中具有重要作用。Objective To investigate the changes in peripheral blood Th17 and CD4＋CD25＋ regulatory T （Treg） cells and their significance among children with hand, foot and mouth disease （HFMD）. Methods Eighty-nine children with HFMD, including 55 cases of common HFMD and 34 cases of severe HFMD, were included in the study; and 30 healthy children were selected as the control group. The percentages of Th17 and CD4^＋CD25^＋ Treg cells in CD4＋ T cells in peripheral blood were determined by flow cytometry. The expression levels of interleukin （IL）-10, transforming growth factor-β （TGF-β）, and IL-17 were measured by enzyme-linked immunosorbent assay. Results Compared with the control group, the cases of common HFMD and severe HFMD had significantly increased levels of Th17 cells and IL-17 （P〈0.05） but significantly decreased levels of CD4^＋CD25^＋ Treg cells, IL-10, and TGF-β （P〈0.05）. The severity of the HFMD was positively correlated with the levels of Th17 cells and IL-17 in peripheral blood but negatively correlated with the levels of CD4^＋CD25^＋ Treg cells, IL-10, and TGF-β. Conclusions Children with HFMD have increased response of Th17 cells but decreased response of CD4^＋CD25^＋ Treg cells in peripheral blood. Th17/CD4^＋CD25^＋ Treg cell imbalance may play an important role in the pathogenesis of HFMD.

关 键 词：手足口病 TH17细胞 CD4^+CD25^+调节性T细胞 儿童 

分 类 号：R725.1[医药卫生—儿科] R446.6[医药卫生—临床医学]