肌肽对小鼠放射性肺损伤的防治作用  

Prevention effect of carnosine on radiation-induced lung injury in mice

作　　者：贾勇士[1] 林白桦[1] 毕爱红[1] 詹文明[1] 张丽萍[1] 许丽萍[1] 李强[1] 

机构地区：[1]浙江省人民医院放疗科,杭州310014

出　　处：《中华放射医学与防护杂志》2013年第6期607-610,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：浙江省医药卫生科技计划项目（2010KYA017）

摘　　要：目的 了解肌肽对小鼠放射性肺损伤的防治作用。方法 近交系C57/BL 雌性小鼠108只，随机数字表法分为对照组、单纯照射组、照射＋肌肽组（15 mg·kg-1·d-1）和单纯肌肽组（15 mg·kg-1·d-1），对照组共18只，其他各组每组30只。采用10 MV X射线经前胸单次照射小鼠肺脏中平面13 Gy，全肺照射。两肌肽组灌胃给予15 mg·kg-1·d-1肌肽，照射前30 min给药1次，以后1 次/d至实验结束，对照组给予相应同体积生理盐水。在照射后7、28和56 d处死后取部分肺组织HE染色，部分肺组织行超氧化物歧化酶（SOD）检测，同时ELISA法测定血清中TGF-β1、TNF-α。结果 受照射组（单纯照射组、照射＋肌肽组）在照射后56 d均表现出不同程度的放射性肺炎病理变化，照射＋给药组较单纯照射组炎症反应明显较轻。各组间不同时段血清中TGF-β1水平差异有统计学意义（F=10.65、3.70、4.04，P〈0.05）；各组间不同时段血清中TNF-α水平差异有统计学意义（F=39.55、53.38、4.01，P〈0.05）；各组间不同时段肺组织中SOD水平差异有统计学意义（F=4.33、4.19、3.34，P〈0.05）。照射＋肌肽组较同期的单纯照射组，肺组织中SOD水平明显较高，血清中TGF-β1、TNF-α 明显降低。结论 肌肽通过保护SOD，降低TGF-β1和TNF-α水平起到预防并减轻小鼠放射性肺损伤的作用。Objective To investigate the protective effect of carnosine on radiation-induced lung injury in mice. Methods A total of 108 C57/BL female mice were randomly divided into 4 groups：control group without treatment, irradiation alone group, irradiation ＋ carnosine group （15 mg·kg-1·d-1）, and carnosine alone group （15 mg·kg-1·d-1）. There were 18 mice in control group and 30 mice in every other group.Whole lung anterior chest was irradiated with a single dose of 13 Gy 10 MV X-rays. The mice were administered with carnosine （15 mg·kg-1·d-1） at 30 minutes before irradiation and then gavaged once a day until the end of the experiment.The control group was given with saline. At 7, 28, and 56 d after irradiation, 6 mice of control group and 10 mice of each other group were killed. A portion of lung tissues were stained with HE and other part of lung tissues were used to detect the levels of SOD. Meanwhile, TGF-β1 and TNF-α in the serum were detected with ELISA. Results Different levels of inflammation factors were expressed in the lung tissues of irradiation group and irradiation ＋ carnosine group at 56 d after irradiation, but the inflammation in the irradiation ＋ carnosine group was significantly lighter than that in the irradiation group. Among （7, 28 and 56 d） after radiation, TGF-β1, TNF-α, and SOD levels in different groups had significant differences. At the same time point after irradiation, the level of SOD in lung tissue of irradiation ＋ carnosine group was significantly higher than that of irradiation group（F=4.33,4.19,3.34,P〈0.05）, but the levels of TGF-β1 and TNF-α in serum were reduced. ConclusionsCarnosine can prevent and inhibit radiation-induced lung injury in mice by increasing SOD and reducing TGF-β1 and TNF-α.

关 键 词：肌肽 肺损伤 防治作用 转化生长因子-Β1 

分 类 号：R818[医药卫生—放射医学]