免疫性血小板减少症患者树突状细胞的分布特点及其活化状态的研究  

Distribution and Activation of Dendritic Cells in Immune Thrombocytopenia Patients

作　　者：周振海[1] 李小银[1] 潘倩影[1] 周燕英[1] 苏畅[1] 李娟[1] 

机构地区：[1]中山大学附属第一医院血液内科,广东广州510080

出　　处：《中国实验血液学杂志》2013年第6期1513-1516,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金项目(编号:81170469);广东省自然科学基金项目(编号:S2011010004618)

摘　　要：免疫性血小板减少症(ITP)患者树突状细胞(DC)存在异常。本研究旨在分析ITP患者DC的分布特点及其活化状态,以进一步明确ITP患者DC异常的机制。将ITP患者分为初诊组、难治组、有效组(完全反应+有效),流式细胞术检测各组患者外周血、骨髓和/或脾脏中髓系树突状细胞(mDC),浆细胞样树突状细胞(pDC)的分布比例,各部位mDC、pDC细胞分化程度、成熟mDC和pDC抗原CD80、CD86的表达。结果表明,各组患者各部位mDC比例均较pDC高,难治组各部位mDC、pDC比例较初诊组、有效组高,且难治组中脾脏mDC比例明显高于其他部位;各组pDC比例各部位差别不明显;各组患者各部位mDC、pDC的CD86的表达较CD80高,各组mDC、pDC的CD80的表达比例均较低,各部位差别不明显;难治组各部位mDC、pDC的CD86表达较初诊组、有效组高,且难治组中脾脏mDC的CD86表达明显高于其他部位。结论:ITP患者mDC、pDC的分布存在异常,脾脏mDC分布比例较高,分化较成熟,脾脏mDC可能与ITP发病较为密切。Defective dendritic cell （DC） functions have been implicated in ITP. The purpose of this study was to investigate the distribution and activation of dendritic cells in immune thrombocytopenia （ITP） patients. ITP patients were divided into 3 groups： the newly diagnosed, refractory and effective treatment group. The distributions of plasmacytoid dendritic cells （ pDC ） and myeloid dendritic cells （ mDC ） in peripheral blood, bone marrow and spleen were detected with flow cytometry. The expression level of CD80 and CD86 on surface of pDC and mDC was also detected with flow cytometry. The results indicated that the percentage of mDC was higher than that of pDC in all sites of all groups. The percentage of mDC and pDC in all site of refractory group was higher than that in newly diagnosed and effective groups ; but the percentage of mDC in spleen of refractory group was obviously higher than that in other sites. The percentage of pDC was no significant different in all groups. The expression level of CD86 in all groups was higher than that of CD80,the expression level of CD80 was lower in mDC and pDC of all groups, but there was no obvious difference in all sites. The CD86 expression in all site of refractory group was higher than that in newly diagnosed and effective treatment groups, while the CD86 expression of mDC in spleen of newly diagnosed group obviouly higher than that in other sites. It is concluded that the distribntion abnormality of mDC and pDC exists in ITP patients, the mDC are more accumulated in spleen, and differentiation of mDC to maturity is more obvious in spleen, spleen-derived mDC significantly express CD86, spleen-derived mDC may play an important role in the pathogenesis of ITP.

关 键 词：免疫性血小板减少症 髓系树突状细胞 浆细胞样树突状细胞 

分 类 号：R558.2[医药卫生—血液循环系统疾病]