异基因骨髓移植并发移植物抗宿主病小鼠体内IL-22的细胞来源  

Source and Effect of IL-22 in Graft Versus Host Disease Mouse after Allogeneic Bone Marrow Transplantation

作　　者：赵恺[1,2] 黄栋[2] 赵冬梅[1] 陈翀[2] 吴庆运[1] 潘彬[2] 曾令宇[1] 李振宇[2] 徐开林[1,2] 

机构地区：[1]徐州医学院移植免疫实验室 [2]徐州医学院附属医院血液科,江苏徐州221002

出　　处：《中国实验血液学杂志》2013年第6期1526-1529,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(编号81200376;81270637);中国博士后科学基金资助项目(编号2012M510141);江苏省"六大人才高峰"资助(编号2012-WSN-082);徐州医学院院长专项人才基金(编号2012KJZ13)

摘　　要：本研究探讨小鼠经异基因骨髓移植后发生移植物抗宿主病(GVHD)时体内IL-22的细胞来源。分别以C57BL/6和BALB/c小鼠为供受鼠,受鼠经致死剂量全身照射后输注供鼠骨髓和脾淋巴细胞悬液,建立GVHD模型。实验分为正常组(normal)、单纯照射组(TBI)、骨髓移植组(BMT)和骨髓联合脾细胞诱导GVHD组(BS)。ELISA法检测小鼠血浆中IL-22的水平;流式细胞术检测IL-22的细胞来源及所属亚群情况。结果表明,BS组小鼠血浆中IL-22水平最高,与正常小鼠相比差异有统计学意义(P<0.01);BS组小鼠脾、淋巴结和外周血中淋巴细胞均可产生IL-22,且IL-22+CD4+T细胞百分率高于IL-22+CD8+T细胞;除Th22细胞外,Th1细胞和Th17细胞也是GVHD小鼠体内IL-22的细胞来源,但以Th22细胞分泌IL-22为主。结论:GVHD小鼠体内可产生高水平的IL-22,其主要来源于IFN-γ-IL-17-IL-22+的Th22细胞。This study was purposed to explore the cellular source of IL-22 in graft versus host disease （GVHD） mouse following allo-geneic bone marrow transplantation. BALB/c and C57BL/6 mice were used as recipients and donors, re- spectively. GVHD model was established by irradiated BABL/c mice inoculated with mixed suspension of C57BL/6 bone marrow cells and splenic lymphocytes. The mice were divided into normal group （normal）, total body irradiated group （TBI）, bone marrow cell-transplanted group（ BMT）, and the combination of bone marrow cell and splenic lymphocytes- induced GVHD group（BS）. The level of IL-22 in plasma was detected by ELISA. The cellular source of IL-22 and IL- 22 ＋ subsets were detected by flow cytometry. The results showed that compared with normal mice, the level of IL-22 in plasma from BS mice was the highest（ P 〈 0.01 ）. All the lymphocytes of spleen, lymph nodes and peripheral blood from BS mice could produce IL-22, in which the percentage of IL-22 ＋ CD4 ＋ T cell was higher than that of IL-22＋ CD8 ＋ T cells. Not only Th22 cells but also Thl and Thl7 cells were the cellular source of IL-22 in GVHD mice. It is concluded that the high level of IL-22 has been found in mice with GVHD, which mainly originates from IFN-γ- IL-17 - IL-22 ＋Th22 cells.

关 键 词：骨髓移植 移植物抗宿主病 T细胞 白介素-22 

分 类 号：R457.7[医药卫生—治疗学]