流式荧光杂交法检测女性妇科门诊人群和体检人群的HPV基因型  

Study on the genotyping of human papillomavirus using a method of flow fluorescence hybridization in women from both gynecological outpatient populations and check-up crowd

作　　者：陈占国[1] 李艳[1] 

机构地区：[1]武汉大学人民医院检验科,湖北武汉430060

出　　处：《中国医药导报》2013年第32期123-126,131,共5页China Medical Herald

基　　金：国家临床重点专科建设项目(编号财社[2010]305号)

摘　　要：目的 比较女性门诊人群和体检人群人乳头瘤病毒（HPV）感染情况及常见基因型的差异.方法 采用流式荧光杂交方法检测2098例门诊人群和同期2730例体检人群女性宫颈脱落细胞标本的HPV分型,分析两类人群的HPV感染率、感染类型、常见亚型以及不同年龄段HPV感染类型的差异.结果 门诊人群和体检人群总感染率为39.37％（826/2098）比17.36％ （474/2730）,其中单一亚型感染为29.79％比14.17％（高危亚型为25.83％比11.76％,低危亚型为3.96％比2.41％）,多重感染为9.58％比3.19％（两重感染占6.82％比2.67％,两重以上感染占2.76％比0.52％）.门诊人群常见的HPV亚型为HPV52 （22.03％）、16（19.37％）、58 （14.65％）、33（7.51％）、56（7.51％）、6（5.21％）、61（4.12％）,体检人群常见的HPV亚型为HPV52（20.46％）、58（15.40％）、61（9.70％）、39（8.02％）、16（7.38％）、68（6.96％）、44（5.27％）.门诊人群和体检人群之间,HPV阳性检出率的差异有统计学意义（x2=292.03,P＜0.01）,且门诊人群多重感染率高于体检人群（x2=6.244,P＜0.05）.两类人群单一亚型感染以31～50岁为主,多重感染以两重和三重感染为主,但门诊人群比体检人群的四重和五重感染多见;体检人群的两重感染率最高是＞40～50岁,而门诊人群的两重感染最高是31～40岁,门诊人群多重感染程度有随年龄增加而加重的趋势.结论 门诊人群的HPV感染率和多重感染率明显高于体检人群,体检人群HPV感染以52和58亚型为主,门诊人群以52、16和58亚型为主,本院女性门诊人群和体检人群HPV感染有一定的区域特征性.Objective To compare the difference of infection and genotype in female outpatient populations and check-up crowd with human papillomavirus （HPV）.Methods 2098 cases of cervical exfoliated cell specimens from gynecological outpatients and 2730 cases of identical specimens from check-up crowd were detected by using flow fluorescence hybridization,and the two groups of the overall incidence of HPV,the type of infection,the common subtypes and the distribution characteristics of different age in HPV genotypes were analyzed.Results The overall incidence of HPV in outpatient populations and check-up crowd was 39.37％ （826/2098） vs 17.36％ （474/2730）,in which a single infection was 29.79％ vs 14.17％ （high-risk subtype：25.83％ vs 11.76％,low-risk subtype：3.96％ vs 2.41％） and multiple infection was 9.58％ vs 3.19％ （double infection：6.82％ vs 6.82％,more than double infection：2.76％ vs 0.52％）.The most common HPV subtypes in outpatient populations were HPV-52 （22.03％）,16 （19.37％）,58 （14.65％）,33 （7.51％）,56 （7.51％）,6 （5.21％）,and 61 （4.12％）,while in check-up crowd they were HPV-52 （20.46％）,58 （15.40％）,61 （9.70％）,39 （8.02％）,16 （7.38％）,68 （6.96％）,and 44 （5.27％）.There was a statistically significant difference in positive rate of HPV between outpatient populations and check-up crowd （x2=292.03,P ＜ 0.01） and the multiple infection rate in outpatient populations was higher than in check-up crowd （x2=6.244,P ＜0.05）.A single subtype infection of two groups was common in women who was 31 to 50 years old,and the main multiple infection was double and triple infection,while there were more quadruple and quintuple infection in outpatient populations than in check-up crowd.Women who had the highest double infection rate was more than 40 to 50 years old in check-up crowd and 31 to 40 years old in outpatient populations.The degree of multiple infection in outpatient populations increased along with th

关 键 词：流式荧光杂交法 人乳头瘤病毒 基因型 感染 

分 类 号：R440[医药卫生—诊断学]