异甘草酸镁对人表皮角质形成细胞炎症因子的产生和外周血白细胞趋化功能的影响  

Effects of Magnesium Isoglycyrrhizinate on Expression of Inflammatory Factors in Human Keratinocytes And Human Leukocytes Chemotaxis

作　　者：李新宇[1] 唐美育[1] 徐兰芳[1] 郑家润[1] 

机构地区：[1]中国医学科学院北京协和医学院皮肤病研究所,江苏南京210042

出　　处：《抗感染药学》2013年第4期267-270,共4页Anti-infection Pharmacy

摘　　要：目的:研究异甘草酸镁对角质形成细胞产生促炎症细胞因子、黏附分子和对白细胞趋化功能的影响,以获得异甘草酸镁治疗炎症性皮肤病的药理学基础。方法:采用2 000 U/mL rhIFN-γ诱导人表皮角质形成细胞HaCaT细胞,用ELISA法检测不同浓度的异甘草酸镁对HaCaT细胞IL-8、IL-6和ICAM-1产生的影响;通过琼脂糖平板法在显微镜下观察药物对10 nmol/L甲酰甲二磺酰-亮氨酰-苯丙氨酸(fMLP)诱引的人白细胞移动距离的影响。结果:异甘草酸镁能剂量依赖性地抑制rhIFN-γ刺激引起的IL-8、IL-6和ICAM-1的产生,其IC50值分别为1.21、7.23和4.17μg/mL;对fMLP诱引的人外周血白细胞趋化功能有明显影响,其抑制作用呈剂量依赖性,IC50值为8.58μg/mL。结论:异甘草酸镁可通过抑制人表皮角质形成细胞IL-8、IL-6、ICAM-1产生和白细胞趋化达到治疗炎症相关性皮肤病的作用。Objective： To study the effects of magnesium isoglycyrrhizinate on the production of pro-inflammatory cytokine and adhesion molecules in human keratinocytes and human leukocytes chemotaxis for understanding the pharmacological basis of magnesium isoglycyrrhizinate in treatment of inflammatory skin disease. Methods：HaCaT keratinocytes were induced by recombinant human IFN-γ（rhIFN-γ） and the production of IL-8, IL-6 and ICAM-1 in HaCaT cells was determined by ELISA. The distances of moving human peripheral leucocytes directed by fMetLeuPhe（fMLP） in argarose gel were observed with microscopy. Results：Magnesium isoglycyrrhizinate could inhibit the production of IL-8, IL-6 and sICAM-1 induced by rhIFN-γ in a dose-dependent pattern, with the 50% inhibitory concentration value（IC50） of 1.21, 7.23 and 4.17 μg/mL, respectively. It also inhibits the leucocytes chemotactic activities in a dose-dependent manner with IC50 of 8.58 μg/mL. Conclusion：Magnesium isoglycyrrhizinate can plays the role in treatment of inflammatory skin disease by inhibiting significantly the production of pro-inflammatory cytokine and adhesion molecules in keratinocytes and leukocytes chemotaxis.

关 键 词：异甘草酸镁 角质形成细胞 rhIFN-γ 炎症性皮肤病 

分 类 号：R969[医药卫生—药理学]