RP-HPLC法测定三黄散瘀巴布剂中五种蒽醌类成分的含量  

Quantitative Determination of Five Anthraquinones in Sanhuang Sanyu Cataplasm by RP-HPLC

作　　者：尹华[1] 章建华[2] 许丛辉[1] 王玲[1] 

机构地区：[1]浙江中医药大学药学院中药标准化研究实验室,浙江杭州310053 [2]浙江中医药大学附属第一医院,浙江杭州310006

出　　处：《中华中医药学刊》2013年第12期2655-2658,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省科技厅科技计划资助项目(2007C23020);浙江省中药现代化专项资金和浙江省中医药管理局资助项目(2006C038)

摘　　要：目的：建立三黄散瘀巴布剂中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的RP-HPLC含量测定方法。方法：反相高效液相色谱法。色谱柱：AgilentZorbaxEclipseXDB-C18（4.6mm×150mm,5μm）;柱温25℃;流动相：甲醇-0.1%磷酸;流速：1.0mL.min-1;检测波长：254nm;进样量：10μL。结果：芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在21.45～214.5ng、15.68～156.8ng、18.77～187.7ng、37.40～374.0ng、11.05～88.42ng范围内呈良好的线性关系,平均加样回收率分别为99.82%（RSD2.6%）、100.5%（RSD1.1%）、101.3%（RSD1.5%）、101.5%（RSD2.4%）、97.07%（RSD0.80%）结论：本法稳定、可靠、操作简便、快速、重现性好,可用于三黄散瘀巴布剂的质量控制。Objective： To develop a RP - HPLC method for the determination of Aloe - emodin, Rhein, Emodin, Chrysophanol, Physcion in Sanhuang Sanyu Cataplasm by RP - HPLC simultaneously. Methods ： The analysis was carried on with a column of Zorbax Eclipse SB -C1s（4.6 mm x 150 mm, 5 μm） by HPLC at 25 ℃. Methanol -0.1% H3PQ was used as mobile phase, the flow rate was 1 mL min- , and the detection wavelength was 254 nm. Result： A good line- arity can be found in the range of 21.45 - 214.5 ng for Aloe - emodin, 15.68 - 156.8 ng for Rhein, 18.77- 187.7 ng for Emodin, 37.40 - 374. 0 ng for Chrysophanol, 11.05 - 88.42 ng for Physcion, the average recovery rate was 99.82% （RSD2.6 %）,.100.5% （RSD 1.1%）, 101.3 % （RSD 1.5 % ）, 101.5 % （RSD 2.4 %）, 97.07% （ RSD 0. 80 % ） respectively. Conclusion ： The method was simple, reliable, accurate and available for the quality control of Sanhuang Sanyu Cataplasm.

关 键 词：三黄散瘀巴布剂 芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 RP-HPLC 含量测定 

分 类 号：R284.3[医药卫生—中药学]