芍药苷对低氧大鼠肺动脉平滑肌细胞凋亡的影响  

Effect of Paeoniflorin on Apoptosis of Rat Pulmonary Artery Smooth Muscle Cells under Hypoxia

作　　者：钱国清[1] 褚金国[1] 李国祥[1] 尹凤英[2] 王良兴[3] 

机构地区：[1]宁波大学医学院附属宁波市第一医院感染科,浙江宁波315010 [2]宁波大学医学院附属宁波市第一医院超声科,浙江宁波315010 [3]温州医学院附属一院呼吸内科,浙江温州325003

出　　处：《中华中医药学刊》2013年第12期2709-2710,I0027,I0028,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(Y2101208);宁波大学医学院附属宁波市第一医院青年启动基金资助项目(2010QN004)

摘　　要：目的:研究芍药苷对低氧大鼠肺动脉平滑肌凋亡的影响及其机制。方法:酶消化法联合组织贴块法培养肺动脉平滑肌细胞后随机分为:正常组、低氧组、低氧加芍药苷(0.2、2和20μmol/L)组。采用CCK-8法检测芍药苷对低氧肺动脉平滑肌细胞生长抑制情况;流式细胞术检测细胞周期;免疫细胞化学染色法检测Bcl-2、Bax蛋白表达。结果:芍药苷对低氧肺动脉平滑肌细胞抑制率与其浓度呈正相关;芍药苷使肺动脉平滑肌细胞凋亡率增加,使肺动脉平滑肌细胞发生G2/M期阻滞;芍药苷抑制肺动脉平滑肌细胞Bcl-2蛋白表达,促进Bax蛋白表达。结论:芍药苷可能通过阻滞细胞周期、调节Bcl-2和Bax蛋白表达,诱导低氧大鼠肺动脉平滑肌细胞凋亡。Objective： To study the effect and mechanism of paeoniflorin on the apoptosis of rat pulmonary artery smooth muscle cells under hypoxia. Methods ： PASMCs were obtained from rat pulmonary arteries using digest and explant methods, divided into normoxia （ N）, hypoxia （ H）, hypoxia ＋ different concentrations of paeoniflorin （0.2,2 and 20 μmol/L） groups. PASMCs activity was measured under cell counting kit -8. Flow cytometry was used to detect the cell cycle of PASMCs. The expressions of Bcl - 2 and Bax were determined by immunocytochemistry. Results： Paeoniflorin can significantly increase the apoptosis rates and induce G2/M phase arrest of PASMCs, can inhibit the expression of Bcl -2,and promote the expression of Bax. Conclusions： Paeoniflorin can inhibit the proliferation of rat PASMCs under hy- poxia, through inducing G2/M phase arrest and regulate the expressions of Bcl - 2 and Bax.

关 键 词：芍药苷 低氧 肺动脉平滑肌细胞 凋亡 

分 类 号：R285.5[医药卫生—中药学]