三氯化铁法致大鼠大脑中动脉闭塞模型的改良研究  

Studies of Middle Cerebral Artery Occlusion Model in Rats Induced by Chloride Ferric

作　　者：包玉龙[1] 范英兰[1] 朱竟赫 刘禾[1] 宋达夫[1] 李显华[1] 杜佳林[1] 

机构地区：[1]辽宁省中医药研究院,辽宁沈阳110034

出　　处：《中华中医药学刊》2013年第12期2754-2756,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省科技厅资助项目(2011408004)

摘　　要：目的:改良三氯化铁法致大鼠大脑中动脉闭塞(MCAO)模型。方法:通过神经功能缺损评分、TTC染色、脑含水量检测和病理观察,对改良三氯化铁法和普通三氯化铁法的栓塞效果进行比较观察。结果:改良血栓可形成与普通血栓相同的MCAO,两组之间神经功能缺损评分、梗死面积、脑含水量无显著性差异。结论:改良三氯化铁法致大鼠大脑中动脉闭塞(MCAO)模型保证了大脑中动脉的完整,开颅窗口比较小且损伤较少,手术操作简单,使操作时间明显缩短,模型成功率显著提高,使模型在试验中可以广泛推广使用。Objective：To use the improved chloride ferric method to induce rat middle cerebral artery occlusion （MCAO） model. Methods：Through the neurological impairment score, TTC staining, cerebral water content detection and pathological observation, chloride ferric method and the general chloride ferric method embolic effect was observed in com- parison. Results ： MCAO was formed by modified thrombosis as the same as ordinary thrombosis, neurological impairment score, infarct size and cerebral water content had no significant difference between the two groups. Conclusion：The MCAO model induced by modified chloride ferric method ensured the integrity of middle cerebral artery, craniotomy window smal- ler and less damage. The operation is simple and the time is significantly shortened. The successful rate of model signifi- cantly is improved, so the model in the experiment can be widely used.

关 键 词：三氯化铁 大鼠 大脑中动脉 闭塞 脑栓塞模型 

分 类 号：R285.5[医药卫生—中药学]