肾衰泻浊丸对UUO模型大鼠肾组织CTGF和α-SMA表达的影响  

Effect of Shenshuai Xiezhuo Pill on Expression of CTGT and α-SMA in Rats with Unilateral Ureteral Obstruction

作　　者：杨馨[1,2] 张佩青[2] 黄彦彬[2] 吕波[2] 刘娜[2] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江省中医研究院,黑龙江哈尔滨150036

出　　处：《中华中医药学刊》2013年第12期2792-2793,I0032,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:探讨中药方剂肾衰泻浊丸对UUO大鼠模型肾组织CTGF和α-SMA表达水平的影响,初步探讨其对肾间质纤维化的作用机制。方法:采用单侧输尿管梗阻致肾间质纤维化大鼠模型。分为假手术组、模型组、肾衰泻浊丸高剂量组、肾衰泻浊丸中剂量组、肾衰泻浊丸低剂量组、洛汀新组,运用免疫组化法检测实验第14、21、28天各组的CTGF和α-SMA的表达水平。结果:不同剂量的肾衰泻浊丸组和洛汀新组可明显下调α-SMA和CTGF在实验大鼠肾小管、间质的表达,与模型组相比有显著性差异(P<0.05),且中剂量组效果最优,与洛汀新组相比有显著性差异(P<0.05)。结论:肾衰泻浊丸可以降低实验大鼠肾组织中α-SMA和CTGF的表达水平,延缓肾间质纤维化的进展。Objective ：To investigate the effect of the Shenshuai Xiezhuo Pill on CTGF and α - SMA of kidney tissue of renal interstitial fibrosis rats caused by unilateral urethral obstruction （ UUO）, and to illustrate the mechanism of Shens- huai Xiezhuo Pill on delaying renal interstitial fibrosis. Methods ： The rats were divided into six groups, including sham - operated group, model control, high, middle and low doses groups of Shenshuai Xiezhuo Pill, and Benazepril group. CTGF andα - SMA expressions in kidney tissues were detected by immunohistochemical method on 14,21,28 days. Results ： Shenshuai Xiezhuo pill can significantly lower expressions of CTGF and a - SMA in tubulointerstitial and had obvious difference with control group. Shenshuai Xiezhuo pill can improve the renal tubulointerstitial pathological lesions. Conclu- s/ons ： Shenshuai Xiezhuo pill can significantly lower expressions of CTGF and α- SMA in tubulointerstitial of the unilater- al urethral obstruction animal model, and delay the progression of the renal interstitial fibrosis.

关 键 词：肾衰泻浊丸 肾间质纤维化 慢性肾衰竭 结缔组织生长因子 Α-平滑肌肌动蛋白 

分 类 号：R285.5[医药卫生—中药学]