兰州地区不同性别、年龄群体中乙型肝炎病毒表面大蛋白与HBVDNA相关性的探讨  

Study on hepatitis B virus large surface protein and HBV-DNA detection and their gender and age differences in Lanzhou area

作　　者：李彩东[1] 吴斌[1] 段正军[1] 田鹏飞[1] 

机构地区：[1]兰州市第二人民医院,甘肃兰州730046

出　　处：《国际检验医学杂志》2013年第B12期10-12,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨乙型肝炎病毒表面大蛋白（HBV-LP）用于临床诊断的价值以及与HBVDNA和丙氨酸氨基转移酶（ALT）的相关性，并分析其在不同性另4、年龄群体中的差异性。方法分．蓦4采用酶联免疫吸附试验（ELISA）、实时荧光定量PCR（RT—PCR）、化学发光法（CLIA）和全自动生化分析仪对1036例兰州地区慢性乙型肝炎病毒感染者血清中HBv．LP、HBVDNA、HBeAg以及ALT进行检测，并且分析比较三者之间的阳性率及相关性。结果HBeAg（＋）模式的HBv-DNA与HBV—LP阳性率比较差异无统计学意义（P〉0．05），不受性别、年龄的影响。HBeAg（-）模式的HBV—DNA与HBv-LP阳性率比较差异有统计学意义（P〈0．05），男性组HBV-DNA阳性率略高于女性组，年长组大于年轻组，年轻群体HBV-LP阳性率略高于年长群体。HBeAg（＋）模式群体中ALT〉50U／L的比例远大于HBeAg（-）模式群体。结论在HBeAg（＋）模式群体中HBV—LP与HBV-DNA检测有较高符合性，HBeAg（＋）模式群体中及年长群体中肝受损程度较明显，在HBeAg（-）模式群体中乙肝病毒大蛋白阳性率高于HBV-DNA。HBV-LP是反映HBeAg（-）乙肝病毒携带者病毒复制情况的血清免疫学指标，同时检测HBV—LP与HBV-DNA会更具有指导意义。Objective To study the value of the hepatitis B virus large suriace protein（HBV-LP） in chmcal diagnosis and its as- sociation with the level of HBV-DNA and ALT,to analyze their difference in gender and age groups. Methods Sera of 1 036 HBV carriers were collected. HBV-DNA level was qualitatively detected by using real-time polymerase chain reaction, HBV-LP was measured by enzyme linked immunosorbent assay（ELISA） and ALT was determined by fully-automatic biochemical analyzer in 1 036 cases of infected serum. Results There was no significant difference between the levels of HBV-DNA and HBV-LP（P^0.05） in HBeAg-positive patients,which was not affected by gender and age. Significant difference of positive rate was observed between HBV-DNA and HBV-LP in HBeAg-negative patients（P〈0.05）. The positive rate of HBV-DNA in males was higher than that of females,in older group which was higher than that of younger. The rate of ALT〉50 U/L in HBeAg-positive patients were higher than that of HBeAg-negative ones. Conclusion There was higher coincidence rate between the levels of HBV-LP and HBV-DNA in HBeAg positive patients. The positive rate of HBV-LP was higher than that of HBV-DNA in HBeAg-negative patients and the liver function of HBeAg-positive patients and the older patients were significantly impaired. HBV-LP is a reliable serological marker which can reflect the replication of HBV in HBeAg-negative patients, Simultaneous detection of HBV-LP and HBV-DNA will be more useful.

关 键 词：乙型肝炎病毒 乙肝病毒大蛋白 年龄和性另4 HBV DNA 相关性 

分 类 号：R512.62[医药卫生—内科学]