羧基丁二酰-β-环糊精微球毛细管柱的制备及其应用于气相色谱  

Preparation of Carboxy-Succinyl-β-Cyclodextrin Microspheres Capillary Column and Its Application to GC

作　　者：李英杰[1] 丁莉[1] 吕仁江[1] 张福祥[1] 徐婷婷[1] 吴婧[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,齐齐哈尔161006

出　　处：《理化检验（化学分册）》2013年第12期1458-1461,1464,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：黑龙江省自然科学基金资助项目(B201218)

摘　　要：以采用文献方法合成的化合物羧基丁二酰爷环糊精微球为固定相，通过溶胶-凝胶技术制备了毛细管气相色谱柱。用此毛细管柱分离了难以分开的2种氨基酸和3种手性药物对映体。对此分离柱的色谱参数进行了测定，5对对映体的分离因子在1．22～3．21之间，分离度在7．05～22．74之间。分离DL-甲硫氨酸的试验中，测得其分离因子的相对标准偏差（n=7）小于0．65％。所有数据表明，该色谱柱分离效果良好，适宜用于气相色谱分析。A capillary chromatographic column was prepared by using carboxysuccinyl-β-cyclodextrin microspheres as stationary phase and applying the sol-gel technique. This novel column was used in the separation of 2 amino acids and enantiomers of 3 chiral drugs which were very hard to separate. Several main parameters of this chromatographic column were determined and the following results were obtained： （1） values of separation factor for 5 pairs of enantiomers were found in the range of 1.22-3. 21, with resolution in the range of 7.05-22. 74; （2） relative standard deviation （n=7） of separation factor （β） of DL-methionine was found to be less than 0. 65%. As a whole, this new column showed high efficiency in separation and was favorable to be used in GC analysis.

关 键 词：羧基丁二酰廿环糊精微球 毛细管色谱柱 氨基酸 手性药物 溶胶-凝胶 气相色谱法 对映体 

分 类 号：O657.7[理学—分析化学]